BPA-Induced Deregulation Of Epigenetic Patterns: Effects On Female Zebrafish Reproduction

Bisphenol A (BPA) is one of the commonest Endocrine Disruptor Compounds worldwide. It interferes with vertebrate reproduction, possibly by inducing deregulation of epigenetic mechanisms. To determine its effects on female reproductive physiology and investigate whether changes in the expression leve...

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Bibliographic Details
Published in:Scientific reports 2016-02, Vol.6 (1), p.21982-21982, Article 21982
Main Authors: Santangeli, Stefania, Maradonna, Francesca, Gioacchini, Giorgia, Cobellis, Gilda, Piccinetti, Chiara Carla, Dalla Valle, Luisa, Carnevali, Oliana
Format: Article
Language:English
Subjects:
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Animals
Apoptosis
Apoptosis - genetics
Autophagy - genetics
Benzhydryl Compounds - pharmacology
Bisphenol A
Chromatin
Deregulation
DNA methylation
Endocrine Disruptors - pharmacology
Epigenesis, Genetic - drug effects
Epigenetics
Female
Females
Follicles
Gene Expression Profiling
Gene Expression Regulation - drug effects
Histology
Humanities and Social Sciences
Immunoprecipitation
Maturation
multidisciplinary
Oogenesis - genetics
Phagocytosis
Phenols - pharmacology
Polymerase chain reaction
Reproduction - drug effects
Reproduction - genetics
Reproductive system
Science
Transcriptome
Zebrafish
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Summary:Bisphenol A (BPA) is one of the commonest Endocrine Disruptor Compounds worldwide. It interferes with vertebrate reproduction, possibly by inducing deregulation of epigenetic mechanisms. To determine its effects on female reproductive physiology and investigate whether changes in the expression levels of genes related to reproduction are caused by histone modifications, BPA concentrations consistent with environmental exposure were administered to zebrafish for three weeks. Effects on oocyte growth and maturation, autophagy and apoptosis processes, histone modifications and DNA methylation were assessed by Real-Time PCR (qPCR), histology and chromatin immunoprecipitation combined with qPCR analysis (ChIP-qPCR). The results showed that 5 μg/L BPA down-regulated oocyte maturation-promoting signals, likely through changes in the chromatin structure mediated by histone modifications and promoted apoptosis in mature follicles. These data indicate that the negative effects of BPA on the female reproductive system may be due to its upstream ability to deregulate epigenetic mechanism.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep21982