Dynamics of bacterial populations during bench‐scale bioremediation of oily seawater and desert soil bioaugmented with coastal microbial mats

• Published in: Microbial biotechnology 2016-03, Vol.9 (2), p.157-171
• Main Authors: Ali, Nidaa, Dashti, Narjes, Salamah, Samar, Sorkhoh, Naser, Al‐Awadhi, Husain, Radwan, Samir
• Format: Article
• Language: English
• Subjects: Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Bacteria - metabolism; Biodegradation, Environmental; Biota; Cluster Analysis; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Environmental Pollutants - metabolism; Flavobacteria; Marinobacter hydrocarbonoclasticus; Molecular Sequence Data; Mycobacterium; Oils - metabolism; Phylogeny; Pseudomonas; RNA, Ribosomal, 16S - genetics; Seawater - microbiology; Sequence Analysis, DNA; Soil Microbiology; Xanthobacter tagetidis
• ISSN: 1751-7915; 1751-7915
• DOI: 10.1111/1751-7915.12326

Summary This study describes a bench‐scale attempt to bioremediate Kuwaiti, oily water and soil samples through bioaugmentation with coastal microbial mats rich in hydrocarbonoclastic bacterioflora. Seawater and desert soil samples were artificially polluted with 1% weathered oil, and bioaugmented with microbial mat suspensions. Oil removal and microbial community dynamics were monitored. In batch cultures, oil removal was more effective in soil than in seawater. Hydrocarbonoclastic bacteria associated with mat samples colonized soil more readily than seawater. The predominant oil degrading bacterium in seawater batches was the autochthonous seawater species Marinobacter hydrocarbonoclasticus. The main oil degraders in the inoculated soil samples, on the other hand, were a mixture of the autochthonous mat and desert soil bacteria; Xanthobacter tagetidis, Pseudomonas geniculata, Olivibacter ginsengisoli and others. More bacterial diversity prevailed in seawater during continuous than batch bioremediation. Out of seven hydrocarbonoclastic bacterial species isolated from those cultures, only one, Mycobacterium chlorophenolicum, was of mat origin. This result too confirms that most of the autochthonous mat bacteria failed to colonize seawater. Also culture‐independent analysis of seawater from continuous cultures revealed high‐bacterial diversity. Many of the bacteria belonged to the Alphaproteobacteria, Flavobacteria and Gammaproteobacteria, and were hydrocarbonoclastic. Optimal biostimulation practices for continuous culture bioremediation of seawater via mat bioaugmentation were adding the highest possible oil concentration as one lot in the beginning of bioremediation, addition of vitamins, and slowing down the seawater flow rate. Bioaugmenting oily desert soil and seawater batches with microbial‐mat suspensions was more effective in bioremediating soil than seawater. Autochthonous, hydrocarbonoclastic bacteria in the mats colonized soil more readily than seawater. Seawater was more effectively bioremediated in continuous cultures by autochthonous seawater bacteria via biostimulation practices.