The Role of 99mTc-Annexin V Apoptosis Scintigraphy in Visualizing Early Stage Glucocorticoid-Induced Femoral Head Osteonecrosis in the Rabbit

• Published in: BioMed research international 2016-01, Vol.2016 (2016), p.1-6
• Main Authors: Zhao, Jianmin, Bai, Zhigang, Li, Qiang, Zhang, Guoliang, Li, Jianbo, Liu, Rui, Wang, Xuemei, Liu, Yu, Wang, Xiaolong, Wang, Lei
• Format: Article
• Language: English
• Subjects: Apoptosis; Cancer therapies; Chemotherapy; Drug dosages; Embolisms; Gangrene; Heart attacks; Hip joint; Hospitals; Nuclear medicine; Pathogenesis; Purchasing groups; Rabbits; Studies
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2016/7067259

Objective. To validate the ability of 99mTc-Annexin V to visualize early stage of glucocorticoid-induced femoral head necrosis by comparing with 99mTc-MDP bone scanning. Methods. Femoral head necrosis was induced in adult New Zealand white rabbits by intramuscular injection of methylprednisolone. 99mTc-Annexin scintigraphy and 99mTc-MDP scans were performed before and 5, 6, and 8 weeks after methylprednisolone administration. Rabbits were sacrificed at various time points and conducted for TUNEL and H&E staining. Results. All methylprednisolone treated animals developed femoral head necrosis; at 8 weeks postinjection, destruction of bone structure was evident in H&E staining, and apoptosis was confirmed by the TUNEL assay. This was matched by 99mTc-Annexin V images, which showed a significant increase in signal over baseline. Serial 99mTc-Annexin V scans revealed that increased 99mTc-Annexin V uptake could be observed in 5 weeks. In contrast, there was no effect on 99mTc-MDP signal until 8 weeks. The TUNEL assay revealed that bone cell apoptosis occurred at 5 weeks. Conclusion. 99mTc-Annexin V is superior to 99mTc-MDP for the early detection of glucocorticoid-induced femoral head necrosis in the rabbit and may be a better strategy for the early detection of glucocorticoid-induced femoral head necrosis in patients.