Antitumor effect of a polysaccharide isolated from Phellinus pullus as an immunostimulant

The antitumor function of fungal polysaccharides is a popular area of interest in the research field due to their high efficiency and low side effects. The main mechanism of fungal polysaccharides is immune enhancement. The polysaccharose (APS-3) was extracted from the fruit body of Phellinus pullus...

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Published in:Biomedical reports 2016-03, Vol.4 (3), p.361-364
Main Authors: YANG, WEIHUA, ZHANG, HENGLAN, JI, MINGYU, PEI, FENGYAN, WANG, YUNSHAN
Format: Article
Language:English
Subjects:
Analysis
Animal models
Anticancer properties
Antigens
antitumor
Antitumor activity
Cancer therapies
Care and treatment
Cell culture
Cell proliferation
Chemotherapy
Cytotoxicity
Dosage
Fungi
Health aspects
immune enhancement
Immune system
Immunostimulants
L-Lactate dehydrogenase
Laboratory animals
Lactate dehydrogenase
Lactic acid
Lymphocytes
Mice
Phellinus pullus
Polysaccharides
Rodents
S180
Sarcoma
Side effects
Tumor cells
Tumors
Wood-decaying fungi
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creator YANG, WEIHUA
ZHANG, HENGLAN
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description The antitumor function of fungal polysaccharides is a popular area of interest in the research field due to their high efficiency and low side effects. The main mechanism of fungal polysaccharides is immune enhancement. The polysaccharose (APS-3) was extracted from the fruit body of Phellinus pullus. The proliferation inhibition to mouse sarcoma 180 (S180) tumor cells was studied by the MTT method. Mice models of transplanted S180 tumor were established and treated with APS-3 to verify the antitumor activity in vivo. Natural killer (NK) and lymphokine-activated killer (LAK) cytotoxicities of the mice were evaluated by the lactate dehydrogenase method. APS-3 can significantly inhibit the proliferation of the S180 cells. Cells could be completely inhibited by 1.6 mg/ml APS-3 after 24 h treatment. After 18 days of treatment, the antitumor rate of the high-dose group was 85.47%. Histopathology detection showed that for the APS-3-treated mice, the tumor cells dissolved, and exhibited a large range of structureless necrotic areas. NK and LAK cytotoxicities of the APS-3 treated mice increased by 61.85 and 56.16%, respectively, compared with the normal control mice. APS-3 can be used as an antitumor agent by way of immune enhancement.
doi_str_mv 10.3892/br.2016.587
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2049-9442
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4774323
source PubMed Central
subjects Analysis
Animal models
Anticancer properties
Antigens
antitumor
Antitumor activity
Cancer therapies
Care and treatment
Cell culture
Cell proliferation
Chemotherapy
Cytotoxicity
Dosage
Fungi
Health aspects
immune enhancement
Immune system
Immunostimulants
L-Lactate dehydrogenase
Laboratory animals
Lactate dehydrogenase
Lactic acid
Lymphocytes
Mice
Phellinus pullus
Polysaccharides
Rodents
S180
Sarcoma
Side effects
Tumor cells
Tumors
Wood-decaying fungi
title Antitumor effect of a polysaccharide isolated from Phellinus pullus as an immunostimulant
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