Molecular Characterization by Using Next-Generation Sequencing of Plasmids Containing blaNDM-7 in Enterobacteriaceae from Calgary, Canada

Enterobacteriaceae with blaNDM-7 are relatively uncommon and had previously been described in Europe, India, the United States, and Japan. This study describes the characteristics of Enterobacteriaceae (Klebsiella pneumoniae [n = 2], Escherichia coli [n = 2], Serratia marcescens [n = 1], and Enterob...

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Bibliographic Details
Published in:Antimicrobial agents and chemotherapy 2016-02, Vol.60 (3), p.1258-1263
Main Authors: Chen, L, Peirano, G, Lynch, T, Chavda, K D, Gregson, D B, Church, D L, Conly, J, Kreiswirth, B N, Pitout, J D
Format: Article
Language:English
Subjects:
Alberta - epidemiology
Bacterial Proteins - genetics
beta-Lactamases
beta-Lactamases - genetics
Enterobacteriaceae
Enterobacteriaceae - drug effects
Enterobacteriaceae - genetics
Enterobacteriaceae Infections - epidemiology
Enterobacteriaceae Infections - microbiology
Female
High-Throughput Nucleotide Sequencing - methods
Hospitals
Humans
Klebsiella pneumoniae - drug effects
Klebsiella pneumoniae - genetics
Male
Mechanisms of Resistance
Microbial Sensitivity Tests
Plasmids
Plasmids - genetics
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Summary:Enterobacteriaceae with blaNDM-7 are relatively uncommon and had previously been described in Europe, India, the United States, and Japan. This study describes the characteristics of Enterobacteriaceae (Klebsiella pneumoniae [n = 2], Escherichia coli [n = 2], Serratia marcescens [n = 1], and Enterobacter hormaechei [n = 1] isolates) with blaNDM-7 obtained from 4 patients from Calgary, Canada, from 2013 to 2014. The 46,161-bp IncX3 plasmids with blaNDM-7 are highly similar to other blaNDM-harboring IncX3 plasmids and, interestingly, showed identical structures within the different isolates. This finding may indicate horizontal transmission within our health region, or it may indicate contact with individuals from areas of endemicity within the hospital setting. Patients infected or colonized with bacteria containing blaNDM-7 IncX3 plasmids generate infection control challenges. Epidemiological and molecular studies are required to better understand the dynamics of transmission, the risk factors, and the reservoirs for bacteria harboring blaNDM-7. To the best of our knowledge, this is the first report of S. marcescens and E. hormaechei with blaNDM-7.
ISSN:0066-4804
1098-6596
DOI:10.1128/AAC.02661-15