Expression analysis of ClpB/Hsp100 gene in faba bean (Vicia faba L.) plants in response to heat stress

Heat stress adversely affects the growth and yield of faba bean crop. Accumulation of ClpB/Hsp100 class of proteins is a critical parameter in induction of acquired heat stress tolerance in plants. Heat-induced expression of ClpB/Hsp100 genes has been noted in diverse plant species. Using primers co...

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Bibliographic Details
Published in:Saudi journal of biological sciences 2016-03, Vol.23 (2), p.243-247
Main Authors: Kumar, Ritesh, Singh, Amit Kumar, Lavania, Dhruv, Siddiqui, Manzer H., Al-Whaibi, Mohamed H., Grover, Anil
Format: Article
Language:English
Subjects:
ClpB
Faba bean
Heat stress
Hsp100
Original
Pollen
Protein expression
Thermotolerance
Transcript induction
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Summary:Heat stress adversely affects the growth and yield of faba bean crop. Accumulation of ClpB/Hsp100 class of proteins is a critical parameter in induction of acquired heat stress tolerance in plants. Heat-induced expression of ClpB/Hsp100 genes has been noted in diverse plant species. Using primers complementary to soybean ClpB/Hsp100 gene, we analyzed the transcript expression profile of faba bean ClpB/Hsp100 gene in leaves of seedlings and flowering plants and in pollen grains. ClpB/Hsp100 protein accumulation profile was analyzed in leaves of faba bean seedlings using Arabidopsis thaliana cytoplasmic Hsp101 antibodies. The transcript and protein levels of faba bean ClpB/Hsp100 were significantly induced in response to heat stress.
ISSN:1319-562X
2213-7106
DOI:10.1016/j.sjbs.2015.03.006