Generating Primary Fibroblast Cultures from Mouse Ear and Tail Tissues

Primary cells are derived directly from tissue and are thought to be more representative of the physiological state of cells in vivo than established cell lines. However, primary cell cultures usually have a finite life span and need to be frequently re-established. Fibroblasts are an easily accessi...

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Bibliographic Details
Published in:Journal of visualized experiments 2016-01 (107)
Main Authors: Khan, Muznah, Gasser, Stephan
Format: Article
Language:English
Subjects:
Animals
Cell Line
Developmental Biology
Ear - anatomy & histology
Fibroblasts - cytology
Mice
Mice, Inbred C57BL
Primary Cell Culture - methods
Tail - cytology
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Summary:Primary cells are derived directly from tissue and are thought to be more representative of the physiological state of cells in vivo than established cell lines. However, primary cell cultures usually have a finite life span and need to be frequently re-established. Fibroblasts are an easily accessible source of primary cells. Here, we discuss a simple and quick experimental procedure to establish primary fibroblast cultures from ears and tails of mice. The protocol can be used to establish primary fibroblast cultures from ears stored at RT for up to 10 days. When the protocol is carefully followed, contaminations are unlikely to occur despite the use of non-sterile tissue stored for extended time in some cases. Fibroblasts proliferate rapidly in culture and can be expanded to substantial numbers before undergoing replicative senescence.
ISSN:1940-087X
1940-087X
DOI:10.3791/53565