Radiation-damage-induced phasing: a case study using UV irradiation with light-emitting diodes

Exposure to X‐rays, high‐intensity visible light or ultraviolet radiation results in alterations to protein structure such as the breakage of disulfide bonds, the loss of electron density at electron‐rich centres and the movement of side chains. These specific changes can be exploited in order to ob...

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Bibliographic Details
Published in:Acta crystallographica. Section D, Structural biology Structural biology, 2016-03, Vol.72 (3), p.395-402
Main Authors: de Sanctis, Daniele, Zubieta, Chloe, Felisaz, Franck, Caserotto, Hugo, Nanao, Max H.
Format: Article
Language:English
Subjects:
Biochemistry, Molecular Biology
Crystallization
Crystallography, X-Ray
disulfide bonds
Disulfides - chemistry
Equipment Design
Insulin - chemistry
Life Sciences
light-emitting diodes
Models, Molecular
phasing
Protein Conformation - radiation effects
radiation damage
Research Papers
RIP
SIR
Structural Biology
ultraviolet light
Ultraviolet Rays
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Summary:Exposure to X‐rays, high‐intensity visible light or ultraviolet radiation results in alterations to protein structure such as the breakage of disulfide bonds, the loss of electron density at electron‐rich centres and the movement of side chains. These specific changes can be exploited in order to obtain phase information. Here, a case study using insulin to illustrate each step of the radiation‐damage‐induced phasing (RIP) method is presented. Unlike a traditional X‐ray‐induced damage step, specific damage is introduced via ultraviolet light‐emitting diodes (UV‐LEDs). In contrast to UV lasers, UV‐LEDs have the advantages of small size, low cost and relative ease of use. A case study of radiation‐damage‐induced phasing is discussed using ultraviolet light‐emitting diodes to induce specific radiation damage.
ISSN:2059-7983
2059-7983
DOI:10.1107/S2059798315021658