Nhumirim virus, a novel flavivirus isolated from mosquitoes from the Pantanal, Brazil

We describe the isolation of a novel flavivirus, isolated from a pool of mosquitoes identified as Culex (Culex) chidesteri collected in 2010 in the Pantanal region of west-central Brazil. The virus is herein designated Nhumirim virus (NHUV) after the name of the ranch from which the mosquito pool wa...

Full description

Saved in:
Bibliographic Details
Published in:Archives of virology 2015-01, Vol.160 (1), p.21-27
Main Authors: Pauvolid-Corrêa, Alex, Solberg, Owen, Couto-Lima, Dinair, Kenney, Joan, Serra-Freire, Nicolau, Brault, Aaron, Nogueira, Rita, Langevin, Stanley, Komar, Nicholas
Format: Article
Language:English
Subjects:
Animals
Arachnids
Arthropods
Biomedical and Life Sciences
Biomedicine
birds
Brazil
Cercopithecus aethiops
Culex
Culex - virology
Culex chidesteri
Dengue fever
Female
Flavivirus
Flavivirus - classification
Flavivirus - isolation & purification
Genomes
Horses
Infectious Diseases
Laboratories
Medical Microbiology
Mosquitoes
Original Article
phylogeny
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
RNA
RNA, Viral - classification
RNA, Viral - genetics
RNA, Viral - isolation & purification
Serology
Taxonomy
Ticks
Tropical diseases
Vero Cells
Virology
Viruses
West Nile virus
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We describe the isolation of a novel flavivirus, isolated from a pool of mosquitoes identified as Culex (Culex) chidesteri collected in 2010 in the Pantanal region of west-central Brazil. The virus is herein designated Nhumirim virus (NHUV) after the name of the ranch from which the mosquito pool was collected. Flavivirus RNA was detected by real-time RT-PCR of homogenized mosquitoes and from the corresponding C6/36 culture supernatant. Based on full-genome sequencing, the virus isolate was genetically distinct from but most closely related to Barkedji virus (BJV), a newly described flavivirus from Senegal. Phylogenetic analysis demonstrated that NHUV grouped with mosquito-borne flaviviruses forming a clade with BJV. This clade may be genetically intermediate between the Culex-borne flaviviruses amplified by birds and the insect-only flaviviruses.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-014-2219-8