Immunological risk assessment and human leukocyte antigen antibody testing in kidney transplantation

Detection of clinically relevant antibodies is performed by cell-based assays that detect direct interaction between the antibodies present in the recipient serum with donor cells (crossmatch tests - CDC and flow cytometry) and tests to detect the presence of HLA antibodies using beads coated with H...

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Bibliographic Details
Published in:Indian journal of nephrology 2016-03, Vol.26 (2), p.80-85
Main Author: Phanish, M K
Format: Article
Language:English
Subjects:
Analysis
Antibodies
Antigens
Automation
Cytotoxicity
Flow cytometry
Health aspects
HLA antigens
Immunoglobulins
Immunology
Kidney transplantation
Medical laboratories
Transplants & implants
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Description
Summary:Detection of clinically relevant antibodies is performed by cell-based assays that detect direct interaction between the antibodies present in the recipient serum with donor cells (crossmatch tests - CDC and flow cytometry) and tests to detect the presence of HLA antibodies using beads coated with HLA antigens either in flow cytometry or in solid phase assay platforms. Pooled antigen panels coated with either affinity - purified HLA Class I (HLA-A, HLA-B, and HLA-C) or HLA Class II (HLA-DR, HLA-DQ, and HLA-DP) protein molecules obtained from multiple cell lines and are used as a screening test for the detection of HLA antibody.
ISSN:0971-4065
1998-3662
DOI:10.4103/0971-4065.167275