Beta-tricalcium phosphate granules improve osteogenesis in vitro and establish innovative osteo-regenerators for bone tissue engineering in vivo

• Published in: Scientific reports 2016-03, Vol.6 (1), p.23367-23367, Article 23367
• Main Authors: Gao, Peng, Zhang, Haoqiang, Liu, Yun, Fan, Bo, Li, Xiaokang, Xiao, Xin, Lan, Pingheng, Li, Minghui, Geng, Lei, Liu, Dong, Yuan, Yulin, Lian, Qin, Lu, Jianxi, Guo, Zheng, Wang, Zhen
• Format: Article
• Language: English
• Subjects: 692/1537/805; 692/308/2171; 692/699/578; Alkaline phosphatase; Animals; Bioreactors; Bone Development; Bone growth; Bones; Calcium phosphates; Calcium Phosphates - pharmacology; Cell proliferation; Collagen; Computed tomography; Femur; Humanities and Social Sciences; In Vitro Techniques; Labeling; Mesenchyme; Mice; Microscopy, Electron, Scanning; multidisciplinary; Osteocalcin; Osteogenesis; Osteogenesis - drug effects; Osteopontin; Periosteum; Phosphatase; Regeneration; Science; Science (multidisciplinary); Stem cells; Tissue Engineering; Transcription factors; Tricalcium phosphate
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep23367

The drawbacks of traditional bone-defect treatments have prompted the exploration of bone tissue engineering. This study aimed to explore suitable β-tricalcium phosphate (β-TCP) granules for bone regeneration and identify an efficient method to establish β-TCP-based osteo-regenerators. β-TCP granules with diameters of 1 mm and 1–2.5 mm were evaluated in vitro . The β-TCP granules with superior osteogenic properties were used to establish in vivo bioreactors, referred to as osteo-regenerators, which were fabricated using two different methods. Improved proliferation of bone mesenchymal stem cells (BMSCs), glucose consumption and ALP activity were observed for 1–2.5 mm β-TCP compared with 1-mm granules (P