Tumour imaging by the detection of fibrin clots in tumour stroma using an anti-fibrin Fab fragment

The diagnosis of early and aggressive types of cancer is important for providing effective cancer therapy. Cancer-induced fibrin clots exist only within lesions. Previously, we developed a monoclonal antibody (clone 102-10) that recognizes insoluble fibrin but not fibrinogen or soluble fibrin and co...

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Bibliographic Details
Published in:Scientific reports 2016-03, Vol.6 (1), p.23613-23613, Article 23613
Main Authors: Obonai, Toshifumi, Fuchigami, Hirobumi, Furuya, Fumiaki, Kozuka, Naoyuki, Yasunaga, Masahiro, Matsumura, Yasuhiro
Format: Article
Language:English
Subjects:
13/51
14/34
631/67/2321
631/67/327
Adenocarcinoma
Animals
Antibodies, Monoclonal - administration & dosage
Antibodies, Monoclonal - pharmacology
Blood coagulation
Cancer
Carcinoma, Pancreatic Ductal - diagnosis
Carcinoma, Pancreatic Ductal - genetics
Carcinoma, Pancreatic Ductal - metabolism
Coagulation
Early Detection of Cancer
Fab
Fibrin
Fibrin - immunology
Fibrinogen
Fibrinolysis
Genetic Engineering
Humanities and Social Sciences
Humans
Invasiveness
Mice
multidisciplinary
Mutation
Neoplasms, Experimental
Pancreas
Pancreatic Neoplasms - genetics
Pancreatic Neoplasms - metabolism
Science
Stroma
Tumor Microenvironment
Tumors
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Summary:The diagnosis of early and aggressive types of cancer is important for providing effective cancer therapy. Cancer-induced fibrin clots exist only within lesions. Previously, we developed a monoclonal antibody (clone 102-10) that recognizes insoluble fibrin but not fibrinogen or soluble fibrin and confirmed that fibrin clots form continuously in various cancers. Here, we describe the development of a Fab fragment probe of clone 102-10 for tumour imaging. The distribution of 102-10 Fab was investigated in genetically engineered mice bearing pancreatic ductal adenocarcinoma (PDAC) and its effect on blood coagulation was examined. Immunohistochemical and ex vivo imaging revealed that 102-10 Fab was distributed selectively in fibrin clots in PDAC tumours 3 h after injection and that it disappeared from the body after 24 h. 102-10 Fab had no influence on blood coagulation or fibrinolysis. Tumour imaging using anti-fibrin Fab may provide a safe and effective method for the diagnosis of invasive cancers by detecting fibrin clots in tumour stroma.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep23613