Cefazolin Irreversibly Inhibits Proliferation and Migration of Human Mesenchymal Stromal Cells

Drugs may have a significant effect on postoperative bone healing by reducing the function of human mesenchymal stromal cells (hMSC) or mature osteoblasts. Although cefazolin is one of the most commonly used antibiotic drugs in arthroplasty to prevent infection worldwide, there is a lack of informat...

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Bibliographic Details
Published in:BioMed research international 2016-01, Vol.2016 (2016), p.1-6
Main Authors: Zilkens, Christoph, Lensing-Höhn, Sabine, Fröbel, Julia, Pilge, Hakan, Krauspe, Rüdiger
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Antibiotics
Bone marrow
Cefazolin
Cefazolin - toxicity
Cell adhesion & migration
Cell Movement - drug effects
Cell Proliferation - drug effects
Cells, Cultured
Cement
Dose-Response Relationship, Drug
Drug dosages
Female
Fracture Healing - drug effects
Health aspects
Humans
Infection control
Infections
Joint surgery
Male
Mesenchymal Stromal Cells - drug effects
Middle Aged
Models, Biological
Short term
Stem cells
Surgery
Young Adult
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Summary:Drugs may have a significant effect on postoperative bone healing by reducing the function of human mesenchymal stromal cells (hMSC) or mature osteoblasts. Although cefazolin is one of the most commonly used antibiotic drugs in arthroplasty to prevent infection worldwide, there is a lack of information regarding how cefazolin affects hMSC and therefore may have an effect on early bone healing. We studied the proliferation and migration capacity of primary hMSC during cefazolin treatment at various doses for up to 3 days, as well as the reversibility of the effects during the subsequent 3 days of culture without the drug. We found a time- and dose-dependent reduction of the proliferation rate and the migratory potential. Tests of whether these effects were reversible revealed that doses ≥250 μg/mL or treatments longer than 24 h irreversibly affected the cells. We are the first to show that application of cefazolin irreversibly inhibits the potential of hMSC for migration to the trauma site and local proliferation. Cefazolin should be administered only at the required dosage and time to prevent periprosthetic infection. If long-term administration is required and delayed bone healing is present, cefazolin application must be considered as a cause of delayed bone healing.
ISSN:2314-6133
2314-6141
DOI:10.1155/2016/2042687