Microsurgery-aided in-situ force probing reveals extensibility and viscoelastic properties of individual stress fibers

Actin-myosin filament bundles (stress fibers) are critical for tension generation and cell shape, but their mechanical properties are difficult to access. Here we propose a novel approach to probe individual peripheral stress fibers in living cells through a microsurgically generated opening in the...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2016-03, Vol.6 (1), p.23722-23722, Article 23722
Main Authors: Labouesse, Céline, Gabella, Chiara, Meister, Jean-Jacques, Vianay, Benoît, Verkhovsky, Alexander B.
Format: Article
Language:English
Subjects:
13
14/63
631/1647/2204
631/1647/277
631/1647/767/2200
Actin
Animals
Cell Line
Cell size
Cytological Techniques
Cytoplasm
Elasticity
Fibers
Humanities and Social Sciences
Life Sciences
Mechanical properties
Microsurgery
multidisciplinary
Myosin
Rats
Science
Stress Fibers - physiology
Substrates
Viscoelasticity
Viscosity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Actin-myosin filament bundles (stress fibers) are critical for tension generation and cell shape, but their mechanical properties are difficult to access. Here we propose a novel approach to probe individual peripheral stress fibers in living cells through a microsurgically generated opening in the cytoplasm. By applying large deformations with a soft cantilever we were able to fully characterize the mechanical response of the fibers and evaluate their tension, extensibility, elastic and viscous properties.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep23722