Compression therapy affects collagen type balance in hypertrophic scar

• Published in: The Journal of surgical research 2016-04, Vol.201 (2), p.299-305
• Main Authors: Tejiram, Shawn, MD, Zhang, Jenny, BS, Travis, Taryn E., MD, Carney, Bonnie C., BS, Alkhalil, Abdulnaser, PhD, Moffatt, Lauren T., PhD, Johnson, Laura S., MD, Shupp, Jeffrey W., MD
• Format: Article
• Language: English
• Subjects: Animals; Burn scar; Cicatrix, Hypertrophic - metabolism; Cicatrix, Hypertrophic - prevention & control; Collagen; Collagen Type I - metabolism; Collagen Type III - metabolism; Compression Bandages; Compression therapy; Fluorescent Antibody Technique; Gene Expression; Hydroxyproline - metabolism; Hypertrophic scar; Male; Pressure; Pressure therapy; Surgery; Swine
• ISSN: 0022-4804; 1095-8673
• DOI: 10.1016/j.jss.2015.10.040

Abstract Background The effects of pressure on hypertrophic scar are poorly understood. Decreased extracellular matrix deposition is hypothesized to contribute to changes observed after pressure therapy. To examine this further, collagen composition was analyzed in a model of pressure therapy in hypertrophic scar. Materials and methods Hypertrophic scars created on red Duroc swine ( n  = 8) received pressure treatment (pressure device mounting and delivery at 30 mm Hg), sham treatment (device mounting and no delivery), or no treatment for 2 wk. Scars were assessed weekly and biopsied for histology, hydroxyproline quantification, and gene expression analysis. Transcription levels of collagen precursors COL1A2 and COL3A1 were quantified using reverse transcription-polymerase chain reaction. Masson trichrome was used for general collagen quantification, whereas immunofluorescence was used for collagen types I and III specific quantification. Results Total collagen quantification using hydroxyproline assay showed a 51.9% decrease after pressure initiation. Masson trichrome staining showed less collagen after 1 ( P