Combining experimental evolution with next-generation sequencing: a powerful tool to study adaptation from standing genetic variation

Evolve and resequence (E&R) is a new approach to investigate the genomic responses to selection during experimental evolution. By using whole genome sequencing of pools of individuals (Pool-Seq), this method can identify selected variants in controlled and replicable experimental settings. Revie...

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Bibliographic Details
Published in:Heredity 2015-05, Vol.114 (5), p.431-440
Main Authors: Schlötterer, C, Kofler, R, Versace, E, Tobler, R, Franssen, S U
Format: Article
Language:English
Subjects:
Adaptation, Physiological - genetics
Animals
Biological Evolution
Drosophila melanogaster - genetics
Evolutionary biology
Experimental design
Gene Frequency
Genetic Association Studies
Genetic diversity
Genetic Variation
Genetics
Genotype
High-Throughput Nucleotide Sequencing
Phenotype
Polymorphism, Single Nucleotide
Research Design
Review
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Summary:Evolve and resequence (E&R) is a new approach to investigate the genomic responses to selection during experimental evolution. By using whole genome sequencing of pools of individuals (Pool-Seq), this method can identify selected variants in controlled and replicable experimental settings. Reviewing the current state of the field, we show that E&R can be powerful enough to identify causative genes and possibly even single-nucleotide polymorphisms. We also discuss how the experimental design and the complexity of the trait could result in a large number of false positive candidates. We suggest experimental and analytical strategies to maximize the power of E&R to uncover the genotype-phenotype link and serve as an important research tool for a broad range of evolutionary questions.
ISSN:0018-067X
1365-2540
DOI:10.1038/hdy.2014.86