Bioengineering a 3D integumentary organ system from iPS cells using an in vivo transplantation model

The integumentary organ system is a complex system that plays important roles in waterproofing, cushioning, protecting deeper tissues, excreting waste, and thermoregulation. We developed a novel in vivo transplantation model designated as a clustering-dependent embryoid body transplantation method a...

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Bibliographic Details
Published in:Science advances 2016-04, Vol.2 (4), p.e1500887-e1500887
Main Authors: Takagi, Ryoji, Ishimaru, Junko, Sugawara, Ayaka, Toyoshima, Koh-Ei, Ishida, Kentaro, Ogawa, Miho, Sakakibara, Kei, Asakawa, Kyosuke, Kashiwakura, Akitoshi, Oshima, Masamitsu, Minamide, Ryohei, Sato, Akio, Yoshitake, Toshihiro, Takeda, Akira, Egusa, Hiroshi, Tsuji, Takashi
Format: Article
Language:English
Subjects:
Animals
Bioengineering
Biomedical Engineering
Hair Follicle - transplantation
Induced Pluripotent Stem Cells - transplantation
Integumentary System - growth & development
Mice
Organ Transplantation - methods
SciAdv r-articles
Sebaceous Glands - transplantation
Skin - growth & development
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Summary:The integumentary organ system is a complex system that plays important roles in waterproofing, cushioning, protecting deeper tissues, excreting waste, and thermoregulation. We developed a novel in vivo transplantation model designated as a clustering-dependent embryoid body transplantation method and generated a bioengineered three-dimensional (3D) integumentary organ system, including appendage organs such as hair follicles and sebaceous glands, from induced pluripotent stem cells. This bioengineered 3D integumentary organ system was fully functional following transplantation into nude mice and could be properly connected to surrounding host tissues, such as the epidermis, arrector pili muscles, and nerve fibers, without tumorigenesis. The bioengineered hair follicles in the 3D integumentary organ system also showed proper hair eruption and hair cycles, including the rearrangement of follicular stem cells and their niches. Potential applications of the 3D integumentary organ system include an in vitro assay system, an animal model alternative, and a bioengineered organ replacement therapy.
ISSN:2375-2548
2375-2548
DOI:10.1126/sciadv.1500887