Molecular surveillance of nasopharyngeal carriage of Streptococcus pneumoniae in children vaccinated with conjugated polysaccharide pneumococcal vaccines

Following the introduction of pneumococcal conjugate vaccines (PCVs) for infants, surveillance studies on Streptococcus pneumoniae carriage have proven valuable for monitoring vaccine effects. Here, we compared molecular versus conventional diagnostic methods in prospective cross-sectional surveilla...

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Bibliographic Details
Published in:Scientific reports 2016-04, Vol.6 (1), p.23809-23809, Article 23809
Main Authors: Wyllie, Anne L., Wijmenga-Monsuur, Alienke J., van Houten, Marlies A., Bosch, Astrid A. T. M., Groot, James A., van Engelsdorp Gastelaars, Jody, Bruin, Jacob P., Bogaert, Debby, Rots, Nynke Y., Sanders, Elisabeth A. M., Trzciński, Krzysztof
Format: Article
Language:English
Subjects:
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Carrier State - microbiology
Child, Preschool
Children
Cross-Sectional Studies
Deoxyribonucleic acid
DNA
DNA, Bacterial - isolation & purification
Humanities and Social Sciences
Humans
Immunization Programs
Infant
Infants
multidisciplinary
Nasopharynx - immunology
Nasopharynx - microbiology
Netherlands
Pneumococcal Infections - microbiology
Pneumococcal Vaccines - administration & dosage
Polymerase Chain Reaction
Polysaccharides
Prospective Studies
Science
Science (multidisciplinary)
Seasons
Serotypes
Serotyping
Streptococcus infections
Streptococcus pneumoniae
Streptococcus pneumoniae - isolation & purification
Surveillance
Vaccines
Vaccines, Conjugate - administration & dosage
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Summary:Following the introduction of pneumococcal conjugate vaccines (PCVs) for infants, surveillance studies on Streptococcus pneumoniae carriage have proven valuable for monitoring vaccine effects. Here, we compared molecular versus conventional diagnostic methods in prospective cross-sectional surveillances in vaccinated infants in the Netherlands. Nasopharyngeal samples (n = 1169) from 11- and 24-month-old children, collected during autumn/winter 2010/2011 and 2012/2013, were tested by conventional culture for S. pneumoniae. DNA extracted from all culture-plate growth was tested by qPCR for pneumococcal-specific genes ( lytA / piaB ) and selected serotypes (including PCV13-serotypes). qPCR significantly increased the number of carriers detected compared to culture (69% vs. 57%, p  
ISSN:2045-2322
2045-2322
DOI:10.1038/srep23809