Methylation Status of SP1 Sites within miR-23a-27a-24-2 Promoter Region Influences Laryngeal Cancer Cell Proliferation and Apoptosis

DNA methylation plays critical roles in regulation of microRNA expression and function. miR-23a-27a-24-2 cluster has various functions and aberrant expression of the cluster is a common event in many cancers. However, whether DNA methylation influences the cluster expression and function is not repo...

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Bibliographic Details
Published in:BioMed research international 2016-01, Vol.2016 (2016), p.1-8
Main Authors: Xu, Zhen-Ming, Qiu, Guang-Bin, Chen, Sheng, Zhang, Zhao-Xiong, Wang, Ye, Fu, Wei-Neng
Format: Article
Language:English
Subjects:
Apoptosis
Apoptosis - genetics
Biomedical research
Bone cancer
Breast cancer
Cancer
Cell growth
Cell Proliferation - genetics
Colorectal cancer
Deoxyribonucleic acid
DNA
DNA methylation
DNA Methylation - genetics
Epigenetics
Gastric cancer
Gene expression
Gene Expression Regulation, Neoplastic
Genes
HEK293 Cells
Humans
Laryngeal cancer
Laryngeal Neoplasms - genetics
Laryngeal Neoplasms - pathology
Leukemia
Liver cancer
Metastasis
Methylation
MicroRNAs - biosynthesis
MicroRNAs - genetics
Prevention
Promoter Regions, Genetic
Sp1 Transcription Factor - genetics
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Summary:DNA methylation plays critical roles in regulation of microRNA expression and function. miR-23a-27a-24-2 cluster has various functions and aberrant expression of the cluster is a common event in many cancers. However, whether DNA methylation influences the cluster expression and function is not reported. Here we found a CG-rich region spanning two SP1 sites in the cluster promoter region. The SP1 sites in the cluster were demethylated and methylated in Hep2 cells and HEK293 cells, respectively. Meanwhile, the cluster was significantly upregulated and downregulated in Hep2 cells and HEK293 cells, respectively. The SP1 sites were remethylated and the cluster was significantly downregulated in Hep2 cells into which methyl donor, S-adenosyl-L-methionine, was introduced. Moreover, S-adenosyl-L-methionine significantly increased Hep2 cell viability and repressed Hep2 cell early apoptosis. We also found that construct with two SP1 sites had highest luciferase activity and SP1 specifically bound the gene cluster promoter in vitro. We conclude that demethylated SP1 sites in miR-23a-27a-24-2 cluster upregulate the cluster expression, leading to proliferation promotion and early apoptosis inhibition in laryngeal cancer cells.
ISSN:2314-6133
2314-6141
DOI:10.1155/2016/2061248