Proton mediated control of biochemical reactions with bioelectronic pH modulation

In Nature, protons (H + ) can mediate metabolic process through enzymatic reactions. Examples include glucose oxidation with glucose dehydrogenase to regulate blood glucose level, alcohol dissolution into carboxylic acid through alcohol dehydrogenase and voltage-regulated H + channels activating bio...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2016-04, Vol.6 (1), p.24080-24080, Article 24080
Main Authors: Deng, Yingxin, Miyake, Takeo, Keene, Scott, Josberger, Erik E., Rolandi, Marco
Format: Article
Language:English
Subjects:
639/166/985
639/166/987
Alcohol
Alcohol dehydrogenase
BASIC BIOLOGICAL SCIENCES
Biochemical Phenomena
Bioluminescence
biomedical engineering
Carboxylic acids
Dehydrogenase
Dehydrogenases
Dissolution
electrical and electronic engineering
Electronics - methods
Glucose
Glucose dehydrogenase
Humanities and Social Sciences
Hydrogen
Hydrogen-Ion Concentration
Luminescent Measurements
multidisciplinary
Oxidation
pH effects
Protons
Science
Signal Processing, Computer-Assisted
Solutions
Transducers
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In Nature, protons (H + ) can mediate metabolic process through enzymatic reactions. Examples include glucose oxidation with glucose dehydrogenase to regulate blood glucose level, alcohol dissolution into carboxylic acid through alcohol dehydrogenase and voltage-regulated H + channels activating bioluminescence in firefly and jellyfish. Artificial devices that control H + currents and H + concentration (pH) are able to actively influence biochemical processes. Here, we demonstrate a biotransducer that monitors and actively regulates pH-responsive enzymatic reactions by monitoring and controlling the flow of H + between PdH x contacts and solution. The present transducer records bistable pH modulation from an “enzymatic flip-flop” circuit that comprises glucose dehydrogenase and alcohol dehydrogenase. The transducer also controls bioluminescence from firefly luciferase by affecting solution pH.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep24080