An FGFR1-SPRY2 Signaling Axis Limits Basal Cell Proliferation in the Steady-State Airway Epithelium

• Published in: Developmental cell 2016-04, Vol.37 (1), p.85-97
• Main Authors: Balasooriya, Gayan I., Johnson, Jo-Anne, Basson, M. Albert, Rawlins, Emma L.
• Format: Article
• Language: English
• Subjects: Animals; Cell Proliferation - physiology; Cells, Cultured; Epithelial Cells - metabolism; Epithelium - metabolism; Female; Intracellular Signaling Peptides and Proteins - genetics; Intracellular Signaling Peptides and Proteins - metabolism; Male; Membrane Proteins - genetics; Membrane Proteins - metabolism; Mice; Mice, Inbred C57BL; Mice, Knockout; Protein Processing, Post-Translational - genetics; Protein Serine-Threonine Kinases; Receptor, Fibroblast Growth Factor, Type 1 - genetics; Receptor, Fibroblast Growth Factor, Type 1 - metabolism; Respiratory Mucosa - cytology; Respiratory Mucosa - metabolism; Signal Transduction; Trachea - cytology; Trachea - metabolism
• ISSN: 1534-5807; 1878-1551
• DOI: 10.1016/j.devcel.2016.03.001

The steady-state airway epithelium has a low rate of stem cell turnover but can nevertheless mount a rapid proliferative response following injury. This suggests a mechanism to restrain proliferation at steady state. One such mechanism has been identified in skeletal muscle in which pro-proliferative FGFR1 signaling is antagonized by SPRY1 to maintain satellite cell quiescence. Surprisingly, we found that deletion of Fgfr1 or Spry2 in basal cells of the adult mouse trachea caused an increase in steady-state proliferation. We show that in airway basal cells, SPRY2 is post-translationally modified in response to FGFR1 signaling. This allows SPRY2 to inhibit intracellular signaling downstream of other receptor tyrosine kinases and restrain basal cell proliferation. An FGFR1-SPRY2 signaling axis has previously been characterized in cell lines in vitro. We now demonstrate an in vivo biological function of this interaction and thus identify an active signaling mechanism that maintains quiescence in the airway epithelium. [Display omitted] •FGFR1 signaling is important for inhibiting airway stem cell proliferation•FGFR1 post-translationally activates SPRY2, which inhibits activity of other RTKs•Balancing the activity of different RTKs tunes airway stem cell proliferation rates•Ciliated cell fate specification also requires FGFR1 but does not require SPRY2 Airway basal cells are highly quiescent at homeostasis. Rawlins et al. show that this quiescence is actively maintained, in part by an FGFR1-SPRY2 signaling mechanism. This FGFR1 signaling limits steady-state basal cell proliferation by repressing an RTK-mediated pro-proliferative signal.