Adsorbing/dissolving Lyoprotectant Matrix Technology for Non-cryogenic Storage of Archival Human Sera

• Published in: Scientific reports 2016-04, Vol.6 (1), p.24186-24186, Article 24186
• Main Authors: Solivio, Morwena J., Less, Rebekah, Rynes, Mathew L., Kramer, Marcus, Aksan, Alptekin
• Format: Article
• Language: English
• Subjects: 639/638/440/951; 692/53/2421; 82; 82/1; 82/51; Biomarkers; Blood Proteins - analysis; Cancer; Dextran; Heterogeneity; Humanities and Social Sciences; Humans; Matrilysin; multidisciplinary; Preservation, Biological - methods; Rehydration; Science; Serum - chemistry; Trehalose; Vitrification
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep24186

Despite abundant research conducted on cancer biomarker discovery and validation, to date, less than two-dozen biomarkers have been approved by the FDA for clinical use. One main reason is attributed to inadvertent use of low quality biospecimens in biomarker research. Most proteinaceous biomarkers are extremely susceptible to pre-analytical factors such as collection, processing and storage. For example, cryogenic storage imposes very harsh chemical, physical and mechanical stresses on biospecimens, significantly compromising sample quality. In this communication, we report the development of an electrospun lyoprotectant matrix and isothermal vitrification methodology for non-cryogenic stabilization and storage of liquid biospecimens. The lyoprotectant matrix was mainly composed of trehalose and dextran (and various low concentration excipients targeting different mechanisms of damage) and it was engineered to minimize heterogeneity during vitrification. The technology was validated using five biomarkers; LDH, CRP, PSA, MMP-7 and C3a. Complete recovery of LDH, CRP and PSA levels was achieved post-rehydration while more than 90% recovery was accomplished for MMP-7 and C3a, showing promise for isothermal vitrification as a safe, efficient and low-cost alternative to cryogenic storage.