Evaluation of in vitro and in vivo biocompatibility of a myo-inositol hexakisphosphate gelated polyaniline hydrogel in a rat model

• Published in: Scientific reports 2016-04, Vol.6 (1), p.23931, Article 23931
• Main Authors: Sun, Kwang-Hsiao, Liu, Zhao, Liu, Changjian, Yu, Tong, Shang, Tao, Huang, Chen, Zhou, Min, Liu, Cheng, Ran, Feng, Li, Yun, Shi, Yi, Pan, Lijia
• Format: Article
• Language: English
• Subjects: 13/106; 13/107; 13/51; 14/63; 639/166/985; 639/301/54/990; 96/1; Adipose Tissue - cytology; Aniline Compounds - chemistry; Animals; Biocompatibility; Biosensors; Cell Adhesion; Cell adhesion & migration; Cell Proliferation; Cells, Cultured; Coated Materials, Biocompatible - adverse effects; Coated Materials, Biocompatible - chemistry; Cytotoxicity; Drug delivery; Electricity; Endothelial Progenitor Cells - drug effects; Endothelial Progenitor Cells - physiology; Growth rate; Humanities and Social Sciences; Hydrogels; Hydrogels - chemistry; Inflammation; Inositol phosphate; Male; Mesenchymal Stem Cells - drug effects; Mesenchymal Stem Cells - physiology; multidisciplinary; Neural stem cells; Phytic Acid - chemistry; Polycaprolactone; Polyesters - chemistry; Probes; Rats; Rats, Sprague-Dawley; Rodents; Science; Science (multidisciplinary); Stem cell transplantation; Tissue engineering; Tissue Scaffolds - adverse effects; Tissue Scaffolds - chemistry
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep23931

Recent advances in understanding the interaction between electricity and cells/biomolecules have generated great interest in developing biocompatible electrically conductive materials. In this study, we investigated the biocompatibility of a myo-inositol hexakisphosphate gelated polyaniline hydrogel using in vitro and in vivo experiments in a rat model. The polyaniline hydrogel was used to coat a polycaprolactone scaffold and was cultured with rat endothelial progenitor cells differentiated from rat adipose-derived stem cells. Compared with the control sample on a pristine polycaprolactone scaffold, the treated polyaniline hydrogel had the same non-poisonous/cytotoxicity grade, enhanced cell adhesion, and a higher cell proliferation/growth rate. In implant studies, the polyaniline hydrogel sample induced milder inflammatory responses than did the control at the same time points. Combining the advantages of a biocompatible hydrogel and an organic conductor, the inositol phosphate-gelated polyaniline hydrogel could be used in bioelectronics applications such as biosensors, neural probes, cell stimulators, medical electrodes, tissue engineering, and electro-controlled drug delivery.