A reliable Raman-spectroscopy-based approach for diagnosis, classification and follow-up of B-cell acute lymphoblastic leukemia

Acute lymphoblastic leukemia type B (B-ALL) is a neoplastic disorder that shows high mortality rates due to immature lymphocyte B-cell proliferation. B-ALL diagnosis requires identification and classification of the leukemia cells. Here, we demonstrate the use of Raman spectroscopy to discriminate n...

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Bibliographic Details
Published in:Scientific reports 2016-04, Vol.6 (1), p.24821-24821, Article 24821
Main Authors: Managò, Stefano, Valente, Carmen, Mirabelli, Peppino, Circolo, Diego, Basile, Filomena, Corda, Daniela, De Luca, Anna Chiara
Format: Article
Language:English
Subjects:
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Acute lymphoblastic leukemia
Antigens
Antineoplastic Agents - therapeutic use
Biomarkers
Cell Differentiation
Cell Line, Tumor
Cell proliferation
Cell surface
Classification
Deoxyribonucleic acid
DNA
Humanities and Social Sciences
Humans
Immunofluorescence
Immunophenotyping
Leukemia
Leukemia, B-Cell - diagnosis
Leukemia, B-Cell - drug therapy
Leukemia, B-Cell - immunology
Leukemia, B-Cell - pathology
Lymphatic leukemia
Lymphocytes
Lymphocytes B
multidisciplinary
Raman spectroscopy
Science
Spectroscopy
Spectrum analysis
Spectrum Analysis, Raman - methods
Surface antigens
Tumor cell lines
Western blotting
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Description
Summary:Acute lymphoblastic leukemia type B (B-ALL) is a neoplastic disorder that shows high mortality rates due to immature lymphocyte B-cell proliferation. B-ALL diagnosis requires identification and classification of the leukemia cells. Here, we demonstrate the use of Raman spectroscopy to discriminate normal lymphocytic B-cells from three different B-leukemia transformed cell lines (i.e., RS4;11, REH, MN60 cells) based on their biochemical features. In combination with immunofluorescence and Western blotting, we show that these Raman markers reflect the relative changes in the potential biological markers from cell surface antigens, cytoplasmic proteins and DNA content and correlate with the lymphoblastic B-cell maturation/differentiation stages. Our study demonstrates the potential of this technique for classification of B-leukemia cells into the different differentiation/maturation stages, as well as for the identification of key biochemical changes under chemotherapeutic treatments. Finally, preliminary results from clinical samples indicate high consistency of and potential applications for, this Raman spectroscopy approach.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep24821