A upconversion luminescene biosensor based on dual-signal amplification for the detection of short DNA species of c-erbB-2 oncogene

High-sensitivity detection of trace amounts of c-erbB-2 oncogene was reported to be equal to or surpass the ability of CA 15-3 for early diagnosis and/or follow-up recurrent screening of breast cancer. Therefore, in the current study, by using upconversion nanoparticles (UCNPs), rare earth-doped NaY...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2016-04, Vol.6 (1), p.24813-24813, Article 24813
Main Authors: Lan, Jianming, Liu, Yingxin, Li, Li, Wen, Fadi, Wu, Fang, Han, Zhizhong, Sun, Weiming, Li, Chunyan, Chen, Jinghua
Format: Article
Language:English
Subjects:
38/71
631/92/147
692/53/2421
Biosensing Techniques
Biosensors
Breast cancer
Concatamers
Deoxyribonucleic acid
DNA
DNA - chemistry
ErbB protein
ErbB-2 protein
Exonuclease
Genes, erbB-2
Humanities and Social Sciences
Humans
Luminescence
Luminescent Measurements - methods
multidisciplinary
Nanoparticles
Nanoparticles - chemistry
Oncogenes
Real-Time Polymerase Chain Reaction
Science
Science (multidisciplinary)
Self-assembly
Sensitivity and Specificity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:High-sensitivity detection of trace amounts of c-erbB-2 oncogene was reported to be equal to or surpass the ability of CA 15-3 for early diagnosis and/or follow-up recurrent screening of breast cancer. Therefore, in the current study, by using upconversion nanoparticles (UCNPs), rare earth-doped NaYF 4 :Yb 3+ /Er 3+ as the luminescent labels, a upconversion luminescent (UCL) biosensor based on dual-signal amplification of exonuclease III (ExoIII)-assisted target cycles and long-range self-assembly DNA concatamers was developed for the detection of c-erbB-2 oncogene. The proposed biosensor exhibited ultrasensitive detection with limit as low as 40 aM, which may express the potential of being used in trace analysis of c-erbB-2 oncogene and early diagnosis of breast cancer.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep24813