The Antitumor Effect of Gekko Sulfated Glycopeptide by Inhibiting bFGF-Induced Lymphangiogenesis

Objective. To study the antilymphangiogenesis effect of Gekko Sulfated Glycopeptide (GSPP) on human lymphatic endothelial cells (hLECs). Methods. MTS was conducted to confirm the antiproliferation effect of GSPP on hLECs; flow cytometry was employed to detect hLECs cycle distribution; the antimigrat...

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Bibliographic Details
Published in:BioMed research international 2016-01, Vol.2016 (2016), p.1-9
Main Authors: Wu, Xiong-zhi, Liu, Chang, Zhu, Cui-hong, Hao, Jian, Man, Ya-Nan, Ding, Xiu-Li, Yang, Xue
Format: Article
Language:English
Subjects:
Analysis
Angiogenesis
Animals
Antineoplastic Agents - administration & dosage
Cancer
Cancer therapies
Cell cycle
Cell growth
Cell Line, Tumor
Colorectal cancer
Development and progression
Extracellular matrix
Fibroblast Growth Factor 2 - metabolism
Fibroblast growth factors
Gekko
Humans
Immunohistochemistry
Kinases
Lymphangiogenesis - drug effects
Male
Medical research
Metastasis
Mice
Mice, Inbred BALB C
Molecular weight
Neoplasms, Experimental - drug therapy
Neoplasms, Experimental - metabolism
Neoplasms, Experimental - pathology
Neovascularization, Pathologic - drug therapy
Neovascularization, Pathologic - metabolism
Neovascularization, Pathologic - pathology
Phosphorylation
Polysaccharides - administration & dosage
Signal transduction
Studies
Sulfates
Treatment Outcome
Tumor Burden - drug effects
Vascular endothelial growth factor
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Summary:Objective. To study the antilymphangiogenesis effect of Gekko Sulfated Glycopeptide (GSPP) on human lymphatic endothelial cells (hLECs). Methods. MTS was conducted to confirm the antiproliferation effect of GSPP on hLECs; flow cytometry was employed to detect hLECs cycle distribution; the antimigration effect of GSPP on hLECs was investigated by wound healing experiment and transwell experiment; tube formation assay was used to examine its inhibitory effect on the lymphangiogenesis; western blotting was conducted to detect the expression of extracellular signal-regulated kinase1/2 (Erk1/2) and p-Erk1/2 after GSPP and basic fibroblast growth factor (bFGF) treatment. Nude mice models were established to investigate the antitumor effect of GSPP in vivo. Decreased lymphangiogenesis caused by GSPP in vivo was verified by immunohistochemical staining. Results. In vitro, GSPP (10 μg/mL, 100 μg/mL) significantly inhibited bFGF-induced hLECs proliferation, migration, and tube-like structure formation (P
ISSN:2314-6133
2314-6141
DOI:10.1155/2016/7396392