Expression of IPT in Asakura-sanshoo (Zanthoxylum piperitum (L.) DC. f. inerme Makino) Alters Tree Architecture, Delays Leaf Senescence, and Changes Leaf Essential Oil Composition

The IPT gene encodes isopentenyl pyrophosphate transferase, a key enzyme in cytokinin biosynthesis. We introduced IPT under the control of the CaMV35S promoter into Asakura-sanshoo ( Zanthoxylum piperitum (L.) DC. f. inerme Makino) via stable Agrobacterium tumefaciens -mediated transformation. Three...

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Bibliographic Details
Published in:Plant molecular biology reporter 2016-06, Vol.34 (3), p.649-658
Main Authors: Zeng, Xiao-Fang, Zhao, De-Gang
Format: Article
Language:English
Subjects:
Agrobacterium
Agrobacterium radiobacter
Aromatic compounds
auxins
Bioinformatics
Biomedical and Life Sciences
Biosynthesis
branching
cytokinins
Essential oils
genes
genetic transformation
growth habit
Leaves
Life Sciences
Metabolomics
Original Paper
phenotype
Plant biology
Plant Breeding/Biotechnology
Plant Sciences
Proteomics
sesquiterpenoids
stem elongation
surface area
transgenic plants
trees
Zanthoxylum
Zanthoxylum piperitum
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Summary:The IPT gene encodes isopentenyl pyrophosphate transferase, a key enzyme in cytokinin biosynthesis. We introduced IPT under the control of the CaMV35S promoter into Asakura-sanshoo ( Zanthoxylum piperitum (L.) DC. f. inerme Makino) via stable Agrobacterium tumefaciens -mediated transformation. Three of 3-year-old transgenic Asakura-sanshoo lines Y5, Y16, and Y17 were used to evaluate the effects of IPT expression on the morphological characteristics, leaf senescence, and essential oil composition. Introduced IPT into Asakura-sanshoo stimulated an increase in cytokinin content and a decrease in auxin level. The increase in the cytokinin/auxin ratio affected the tree architecture in 3-year-old transgenic lines. The phenotypes of transgenic lines included reduced stem elongation, decreased leaf surface area, increased branching, and delayed leaf senescence. The expression of IPT in Asakura-sanshoo also affected the leaf essential oil composition. The amount of oxygenated sesquiterpenoid compounds in Y5 and Y16 was 21.1 and 15.8 % higher, respectively, than that in wild type (WT). The amount of aromatic compounds in Y5 and Y16 was 2.9 and 24.6 % lower, respectively, than that in WT. These results show that ipt expression in Asakura-sanshoo conferred desirable traits, including a dwarf growth habit, delayed senescence, and increased concentrations of some sesquiterpenoid compounds.
ISSN:0735-9640
1572-9818
1556-469X
DOI:10.1007/s11105-015-0948-9