Loading…
Expression of IPT in Asakura-sanshoo (Zanthoxylum piperitum (L.) DC. f. inerme Makino) Alters Tree Architecture, Delays Leaf Senescence, and Changes Leaf Essential Oil Composition
The IPT gene encodes isopentenyl pyrophosphate transferase, a key enzyme in cytokinin biosynthesis. We introduced IPT under the control of the CaMV35S promoter into Asakura-sanshoo ( Zanthoxylum piperitum (L.) DC. f. inerme Makino) via stable Agrobacterium tumefaciens -mediated transformation. Three...
Saved in:
Published in: | Plant molecular biology reporter 2016-06, Vol.34 (3), p.649-658 |
---|---|
Main Authors: | , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c602t-60b2482f750f29475f0f6d2b53b1f75b6ad092d23147367937ce59e084acbaca3 |
---|---|
cites | cdi_FETCH-LOGICAL-c602t-60b2482f750f29475f0f6d2b53b1f75b6ad092d23147367937ce59e084acbaca3 |
container_end_page | 658 |
container_issue | 3 |
container_start_page | 649 |
container_title | Plant molecular biology reporter |
container_volume | 34 |
creator | Zeng, Xiao-Fang Zhao, De-Gang |
description | The
IPT
gene encodes isopentenyl pyrophosphate transferase, a key enzyme in cytokinin biosynthesis. We introduced
IPT
under the control of the CaMV35S promoter into Asakura-sanshoo (
Zanthoxylum piperitum
(L.) DC. f.
inerme
Makino) via stable
Agrobacterium tumefaciens
-mediated transformation. Three of 3-year-old transgenic Asakura-sanshoo lines Y5, Y16, and Y17 were used to evaluate the effects of
IPT
expression on the morphological characteristics, leaf senescence, and essential oil composition. Introduced
IPT
into Asakura-sanshoo stimulated an increase in cytokinin content and a decrease in auxin level. The increase in the cytokinin/auxin ratio affected the tree architecture in 3-year-old transgenic lines. The phenotypes of transgenic lines included reduced stem elongation, decreased leaf surface area, increased branching, and delayed leaf senescence. The expression of
IPT
in Asakura-sanshoo also affected the leaf essential oil composition. The amount of oxygenated sesquiterpenoid compounds in Y5 and Y16 was 21.1 and 15.8 % higher, respectively, than that in wild type (WT). The amount of aromatic compounds in Y5 and Y16 was 2.9 and 24.6 % lower, respectively, than that in WT. These results show that
ipt
expression in Asakura-sanshoo conferred desirable traits, including a dwarf growth habit, delayed senescence, and increased concentrations of some sesquiterpenoid compounds. |
doi_str_mv | 10.1007/s11105-015-0948-9 |
format | article |
fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4848336</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1836652566</sourcerecordid><originalsourceid>FETCH-LOGICAL-c602t-60b2482f750f29475f0f6d2b53b1f75b6ad092d23147367937ce59e084acbaca3</originalsourceid><addsrcrecordid>eNqNUstuEzEUHSEQDYEPYIMssUklJvgxfsymUpQGqBRUJMKGjeWZ3GncztiDPYOa7-IHcZVQFSSkLixb955z7sMny14TPCcYy_eREIJ5jkk6ZaHy8kk2IVzSvFREPc0mWDKel6LAJ9mLGK9x4mClnmcnVBJFCZaT7Nfqtg8Qo_UO-QZdfNkg69AimpsxmDwaF3feo9l344adv923Y4d620OwQ3rN1vNTdL6co2aeWBA6QJ_NjXX-FC3aAUJEmwCAFqHe2QHqYQzwDp1Da_YRrcE06Cs4iDW4OsWN26LlzrgrOCZXMYIbrGnRpW3R0ne9j3ZIjb7MnjWmjfDqeE-zbx9Wm-WnfH358WK5WOe1wHTIBa5ooWgjOW5oWUje4EZsacVZRVKwEmaLS7qljBSSCVkyWQMvAavC1JWpDZtmZwfdfqw62KY-h2Ba3QfbmbDX3lj9d8bZnb7yP3WhCsWYSAKzo0DwP0aIg-5sGrdtjQM_Rk0UE4JTLh4DpUIoIVXxCCjmHGPKWYK-_Qd67cfg0tI0SVJCMpZ6mGbkgKqDjzFAcz8iwfrOaPpgNJ2Mpu-MpsvEefNwN_eMP85KAHoAxJRKnxoelP6v6m9xaN20</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1784673318</pqid></control><display><type>article</type><title>Expression of IPT in Asakura-sanshoo (Zanthoxylum piperitum (L.) DC. f. inerme Makino) Alters Tree Architecture, Delays Leaf Senescence, and Changes Leaf Essential Oil Composition</title><source>Springer Link</source><creator>Zeng, Xiao-Fang ; Zhao, De-Gang</creator><creatorcontrib>Zeng, Xiao-Fang ; Zhao, De-Gang</creatorcontrib><description>The
IPT
gene encodes isopentenyl pyrophosphate transferase, a key enzyme in cytokinin biosynthesis. We introduced
IPT
under the control of the CaMV35S promoter into Asakura-sanshoo (
Zanthoxylum piperitum
(L.) DC. f.
inerme
Makino) via stable
Agrobacterium tumefaciens
-mediated transformation. Three of 3-year-old transgenic Asakura-sanshoo lines Y5, Y16, and Y17 were used to evaluate the effects of
IPT
expression on the morphological characteristics, leaf senescence, and essential oil composition. Introduced
IPT
into Asakura-sanshoo stimulated an increase in cytokinin content and a decrease in auxin level. The increase in the cytokinin/auxin ratio affected the tree architecture in 3-year-old transgenic lines. The phenotypes of transgenic lines included reduced stem elongation, decreased leaf surface area, increased branching, and delayed leaf senescence. The expression of
IPT
in Asakura-sanshoo also affected the leaf essential oil composition. The amount of oxygenated sesquiterpenoid compounds in Y5 and Y16 was 21.1 and 15.8 % higher, respectively, than that in wild type (WT). The amount of aromatic compounds in Y5 and Y16 was 2.9 and 24.6 % lower, respectively, than that in WT. These results show that
ipt
expression in Asakura-sanshoo conferred desirable traits, including a dwarf growth habit, delayed senescence, and increased concentrations of some sesquiterpenoid compounds.</description><identifier>ISSN: 0735-9640</identifier><identifier>EISSN: 1572-9818</identifier><identifier>EISSN: 1556-469X</identifier><identifier>DOI: 10.1007/s11105-015-0948-9</identifier><identifier>PMID: 27182107</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Agrobacterium ; Agrobacterium radiobacter ; Aromatic compounds ; auxins ; Bioinformatics ; Biomedical and Life Sciences ; Biosynthesis ; branching ; cytokinins ; Essential oils ; genes ; genetic transformation ; growth habit ; Leaves ; Life Sciences ; Metabolomics ; Original Paper ; phenotype ; Plant biology ; Plant Breeding/Biotechnology ; Plant Sciences ; Proteomics ; sesquiterpenoids ; stem elongation ; surface area ; transgenic plants ; trees ; Zanthoxylum ; Zanthoxylum piperitum</subject><ispartof>Plant molecular biology reporter, 2016-06, Vol.34 (3), p.649-658</ispartof><rights>The Author(s) 2015</rights><rights>Springer Science+Business Media New York 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c602t-60b2482f750f29475f0f6d2b53b1f75b6ad092d23147367937ce59e084acbaca3</citedby><cites>FETCH-LOGICAL-c602t-60b2482f750f29475f0f6d2b53b1f75b6ad092d23147367937ce59e084acbaca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27182107$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zeng, Xiao-Fang</creatorcontrib><creatorcontrib>Zhao, De-Gang</creatorcontrib><title>Expression of IPT in Asakura-sanshoo (Zanthoxylum piperitum (L.) DC. f. inerme Makino) Alters Tree Architecture, Delays Leaf Senescence, and Changes Leaf Essential Oil Composition</title><title>Plant molecular biology reporter</title><addtitle>Plant Mol Biol Rep</addtitle><addtitle>Plant Mol Biol Report</addtitle><description>The
IPT
gene encodes isopentenyl pyrophosphate transferase, a key enzyme in cytokinin biosynthesis. We introduced
IPT
under the control of the CaMV35S promoter into Asakura-sanshoo (
Zanthoxylum piperitum
(L.) DC. f.
inerme
Makino) via stable
Agrobacterium tumefaciens
-mediated transformation. Three of 3-year-old transgenic Asakura-sanshoo lines Y5, Y16, and Y17 were used to evaluate the effects of
IPT
expression on the morphological characteristics, leaf senescence, and essential oil composition. Introduced
IPT
into Asakura-sanshoo stimulated an increase in cytokinin content and a decrease in auxin level. The increase in the cytokinin/auxin ratio affected the tree architecture in 3-year-old transgenic lines. The phenotypes of transgenic lines included reduced stem elongation, decreased leaf surface area, increased branching, and delayed leaf senescence. The expression of
IPT
in Asakura-sanshoo also affected the leaf essential oil composition. The amount of oxygenated sesquiterpenoid compounds in Y5 and Y16 was 21.1 and 15.8 % higher, respectively, than that in wild type (WT). The amount of aromatic compounds in Y5 and Y16 was 2.9 and 24.6 % lower, respectively, than that in WT. These results show that
ipt
expression in Asakura-sanshoo conferred desirable traits, including a dwarf growth habit, delayed senescence, and increased concentrations of some sesquiterpenoid compounds.</description><subject>Agrobacterium</subject><subject>Agrobacterium radiobacter</subject><subject>Aromatic compounds</subject><subject>auxins</subject><subject>Bioinformatics</subject><subject>Biomedical and Life Sciences</subject><subject>Biosynthesis</subject><subject>branching</subject><subject>cytokinins</subject><subject>Essential oils</subject><subject>genes</subject><subject>genetic transformation</subject><subject>growth habit</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Metabolomics</subject><subject>Original Paper</subject><subject>phenotype</subject><subject>Plant biology</subject><subject>Plant Breeding/Biotechnology</subject><subject>Plant Sciences</subject><subject>Proteomics</subject><subject>sesquiterpenoids</subject><subject>stem elongation</subject><subject>surface area</subject><subject>transgenic plants</subject><subject>trees</subject><subject>Zanthoxylum</subject><subject>Zanthoxylum piperitum</subject><issn>0735-9640</issn><issn>1572-9818</issn><issn>1556-469X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNqNUstuEzEUHSEQDYEPYIMssUklJvgxfsymUpQGqBRUJMKGjeWZ3GncztiDPYOa7-IHcZVQFSSkLixb955z7sMny14TPCcYy_eREIJ5jkk6ZaHy8kk2IVzSvFREPc0mWDKel6LAJ9mLGK9x4mClnmcnVBJFCZaT7Nfqtg8Qo_UO-QZdfNkg69AimpsxmDwaF3feo9l344adv923Y4d620OwQ3rN1vNTdL6co2aeWBA6QJ_NjXX-FC3aAUJEmwCAFqHe2QHqYQzwDp1Da_YRrcE06Cs4iDW4OsWN26LlzrgrOCZXMYIbrGnRpW3R0ne9j3ZIjb7MnjWmjfDqeE-zbx9Wm-WnfH358WK5WOe1wHTIBa5ooWgjOW5oWUje4EZsacVZRVKwEmaLS7qljBSSCVkyWQMvAavC1JWpDZtmZwfdfqw62KY-h2Ba3QfbmbDX3lj9d8bZnb7yP3WhCsWYSAKzo0DwP0aIg-5sGrdtjQM_Rk0UE4JTLh4DpUIoIVXxCCjmHGPKWYK-_Qd67cfg0tI0SVJCMpZ6mGbkgKqDjzFAcz8iwfrOaPpgNJ2Mpu-MpsvEefNwN_eMP85KAHoAxJRKnxoelP6v6m9xaN20</recordid><startdate>20160601</startdate><enddate>20160601</enddate><creator>Zeng, Xiao-Fang</creator><creator>Zhao, De-Gang</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope></search><sort><creationdate>20160601</creationdate><title>Expression of IPT in Asakura-sanshoo (Zanthoxylum piperitum (L.) DC. f. inerme Makino) Alters Tree Architecture, Delays Leaf Senescence, and Changes Leaf Essential Oil Composition</title><author>Zeng, Xiao-Fang ; Zhao, De-Gang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c602t-60b2482f750f29475f0f6d2b53b1f75b6ad092d23147367937ce59e084acbaca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Agrobacterium</topic><topic>Agrobacterium radiobacter</topic><topic>Aromatic compounds</topic><topic>auxins</topic><topic>Bioinformatics</topic><topic>Biomedical and Life Sciences</topic><topic>Biosynthesis</topic><topic>branching</topic><topic>cytokinins</topic><topic>Essential oils</topic><topic>genes</topic><topic>genetic transformation</topic><topic>growth habit</topic><topic>Leaves</topic><topic>Life Sciences</topic><topic>Metabolomics</topic><topic>Original Paper</topic><topic>phenotype</topic><topic>Plant biology</topic><topic>Plant Breeding/Biotechnology</topic><topic>Plant Sciences</topic><topic>Proteomics</topic><topic>sesquiterpenoids</topic><topic>stem elongation</topic><topic>surface area</topic><topic>transgenic plants</topic><topic>trees</topic><topic>Zanthoxylum</topic><topic>Zanthoxylum piperitum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zeng, Xiao-Fang</creatorcontrib><creatorcontrib>Zhao, De-Gang</creatorcontrib><collection>SpringerOpen</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Agriculture & Environmental Science Database</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant molecular biology reporter</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zeng, Xiao-Fang</au><au>Zhao, De-Gang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of IPT in Asakura-sanshoo (Zanthoxylum piperitum (L.) DC. f. inerme Makino) Alters Tree Architecture, Delays Leaf Senescence, and Changes Leaf Essential Oil Composition</atitle><jtitle>Plant molecular biology reporter</jtitle><stitle>Plant Mol Biol Rep</stitle><addtitle>Plant Mol Biol Report</addtitle><date>2016-06-01</date><risdate>2016</risdate><volume>34</volume><issue>3</issue><spage>649</spage><epage>658</epage><pages>649-658</pages><issn>0735-9640</issn><eissn>1572-9818</eissn><eissn>1556-469X</eissn><abstract>The
IPT
gene encodes isopentenyl pyrophosphate transferase, a key enzyme in cytokinin biosynthesis. We introduced
IPT
under the control of the CaMV35S promoter into Asakura-sanshoo (
Zanthoxylum piperitum
(L.) DC. f.
inerme
Makino) via stable
Agrobacterium tumefaciens
-mediated transformation. Three of 3-year-old transgenic Asakura-sanshoo lines Y5, Y16, and Y17 were used to evaluate the effects of
IPT
expression on the morphological characteristics, leaf senescence, and essential oil composition. Introduced
IPT
into Asakura-sanshoo stimulated an increase in cytokinin content and a decrease in auxin level. The increase in the cytokinin/auxin ratio affected the tree architecture in 3-year-old transgenic lines. The phenotypes of transgenic lines included reduced stem elongation, decreased leaf surface area, increased branching, and delayed leaf senescence. The expression of
IPT
in Asakura-sanshoo also affected the leaf essential oil composition. The amount of oxygenated sesquiterpenoid compounds in Y5 and Y16 was 21.1 and 15.8 % higher, respectively, than that in wild type (WT). The amount of aromatic compounds in Y5 and Y16 was 2.9 and 24.6 % lower, respectively, than that in WT. These results show that
ipt
expression in Asakura-sanshoo conferred desirable traits, including a dwarf growth habit, delayed senescence, and increased concentrations of some sesquiterpenoid compounds.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>27182107</pmid><doi>10.1007/s11105-015-0948-9</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0735-9640 |
ispartof | Plant molecular biology reporter, 2016-06, Vol.34 (3), p.649-658 |
issn | 0735-9640 1572-9818 1556-469X |
language | eng |
recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4848336 |
source | Springer Link |
subjects | Agrobacterium Agrobacterium radiobacter Aromatic compounds auxins Bioinformatics Biomedical and Life Sciences Biosynthesis branching cytokinins Essential oils genes genetic transformation growth habit Leaves Life Sciences Metabolomics Original Paper phenotype Plant biology Plant Breeding/Biotechnology Plant Sciences Proteomics sesquiterpenoids stem elongation surface area transgenic plants trees Zanthoxylum Zanthoxylum piperitum |
title | Expression of IPT in Asakura-sanshoo (Zanthoxylum piperitum (L.) DC. f. inerme Makino) Alters Tree Architecture, Delays Leaf Senescence, and Changes Leaf Essential Oil Composition |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T12%3A00%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20of%20IPT%20in%20Asakura-sanshoo%20(Zanthoxylum%20piperitum%20(L.)%20DC.%20f.%20inerme%20Makino)%20Alters%20Tree%20Architecture,%20Delays%20Leaf%20Senescence,%20and%20Changes%20Leaf%20Essential%20Oil%20Composition&rft.jtitle=Plant%20molecular%20biology%20reporter&rft.au=Zeng,%20Xiao-Fang&rft.date=2016-06-01&rft.volume=34&rft.issue=3&rft.spage=649&rft.epage=658&rft.pages=649-658&rft.issn=0735-9640&rft.eissn=1572-9818&rft_id=info:doi/10.1007/s11105-015-0948-9&rft_dat=%3Cproquest_pubme%3E1836652566%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c602t-60b2482f750f29475f0f6d2b53b1f75b6ad092d23147367937ce59e084acbaca3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1784673318&rft_id=info:pmid/27182107&rfr_iscdi=true |