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Activation‐Induced Killer Cell Immunoglobulin‐like Receptor 3DL2 Binding to HLA–B27 Licenses Pathogenic T Cell Differentiation in Spondyloarthritis

Objective In the spondyloarthritides (SpA), increased numbers of CD4+ T cells express killer cell immunoglobulin‐like receptor 3DL2 (KIR‐3DL2). The aim of this study was to determine the factors that induce KIR‐3DL2 expression, and to characterize the relationship between HLA–B27 and the phenotype a...

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Published in:Arthritis & rheumatology (Hoboken, N.J.) N.J.), 2016-04, Vol.68 (4), p.901-914
Main Authors: Ridley, Anna, Hatano, Hiroko, Wong‐Baeza, Isabel, Shaw, Jacqueline, Matthews, Katherine K., Al‐Mossawi, Hussein, Ladell, Kristin, Price, David A., Bowness, Paul, Kollnberger, Simon
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container_title Arthritis & rheumatology (Hoboken, N.J.)
container_volume 68
creator Ridley, Anna
Hatano, Hiroko
Wong‐Baeza, Isabel
Shaw, Jacqueline
Matthews, Katherine K.
Al‐Mossawi, Hussein
Ladell, Kristin
Price, David A.
Bowness, Paul
Kollnberger, Simon
description Objective In the spondyloarthritides (SpA), increased numbers of CD4+ T cells express killer cell immunoglobulin‐like receptor 3DL2 (KIR‐3DL2). The aim of this study was to determine the factors that induce KIR‐3DL2 expression, and to characterize the relationship between HLA–B27 and the phenotype and function of KIR‐3DL2–expressing CD4+ T cells in SpA. Methods In total, 34 B27+ patients with SpA, 28 age‐ and sex‐matched healthy controls (20 B27− and 8 B27+), and 9 patients with rheumatoid arthritis were studied. KIR-3DL2 expression and other phenotypic characteristics of peripheral blood and synovial fluid CD4+ T cells were studied by flow cytometry, quantitative polymerase chain reaction, and Western blotting. T cell receptor clonality was determined by template‐switch anchored reverse transcription–polymerase chain reaction and sequencing analysis. Cytokines were measured by enzyme‐linked immunosorbent assay. Results Cellular activation induced KIR‐3DL2 expression on both naive and effector CD4+ T cells. KIR‐3DL2 binding to B27+ cells promoted expression of KIR‐3DL2, the Th17‐specific transcription factor retinoic acid receptor–related orphan nuclear receptor γt, and the antiapoptotic factor B cell lymphoma 2. KIR‐3DL2+CD4+ T cells in patients with ankylosing spondylitis were oligoclonal and enriched for markers of T cell activation and for the gut homing receptor CCR9. In the presence of B27+ antigen‐presenting cells, KIR‐3DL2+CD4+ T cells produced less interleukin‐2 (IL‐2) but more IL‐17. This effect was blocked by HC10, an antibody that inhibits the binding of KIR‐3DL2 to B27 heavy chains. Conclusion KIR‐3DL2 binding to HLA–B27 licenses Th17 cell differentiation in SpA. These findings raise the therapeutic potential of targeting HLA–B27–KIR‐3DL2 interactions for the treatment of B27+ patients with SpA.
doi_str_mv 10.1002/art.39515
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The aim of this study was to determine the factors that induce KIR‐3DL2 expression, and to characterize the relationship between HLA–B27 and the phenotype and function of KIR‐3DL2–expressing CD4+ T cells in SpA. Methods In total, 34 B27+ patients with SpA, 28 age‐ and sex‐matched healthy controls (20 B27− and 8 B27+), and 9 patients with rheumatoid arthritis were studied. KIR-3DL2 expression and other phenotypic characteristics of peripheral blood and synovial fluid CD4+ T cells were studied by flow cytometry, quantitative polymerase chain reaction, and Western blotting. T cell receptor clonality was determined by template‐switch anchored reverse transcription–polymerase chain reaction and sequencing analysis. Cytokines were measured by enzyme‐linked immunosorbent assay. Results Cellular activation induced KIR‐3DL2 expression on both naive and effector CD4+ T cells. KIR‐3DL2 binding to B27+ cells promoted expression of KIR‐3DL2, the Th17‐specific transcription factor retinoic acid receptor–related orphan nuclear receptor γt, and the antiapoptotic factor B cell lymphoma 2. KIR‐3DL2+CD4+ T cells in patients with ankylosing spondylitis were oligoclonal and enriched for markers of T cell activation and for the gut homing receptor CCR9. In the presence of B27+ antigen‐presenting cells, KIR‐3DL2+CD4+ T cells produced less interleukin‐2 (IL‐2) but more IL‐17. This effect was blocked by HC10, an antibody that inhibits the binding of KIR‐3DL2 to B27 heavy chains. Conclusion KIR‐3DL2 binding to HLA–B27 licenses Th17 cell differentiation in SpA. These findings raise the therapeutic potential of targeting HLA–B27–KIR‐3DL2 interactions for the treatment of B27+ patients with SpA.</description><identifier>ISSN: 2326-5191</identifier><identifier>EISSN: 2326-5205</identifier><identifier>DOI: 10.1002/art.39515</identifier><identifier>PMID: 26841353</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Adult ; Aged ; Arthritis, Psoriatic - immunology ; Arthritis, Reactive - immunology ; Arthritis, Rheumatoid - immunology ; Blotting, Western ; Case-Control Studies ; CD4-Positive T-Lymphocytes - immunology ; Cell Differentiation - immunology ; Cytokines - immunology ; Enzyme-Linked Immunosorbent Assay ; Female ; Flow Cytometry ; HLA-B27 Antigen - immunology ; HLA-B27 Antigen - metabolism ; Humans ; Immunoglobulins ; Lymphocytes ; Male ; Middle Aged ; Polymerase chain reaction ; Receptors, Antigen, T-Cell - genetics ; Receptors, KIR3DL2 - immunology ; Receptors, KIR3DL2 - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Spondylarthropathies - immunology ; Spondylitis, Ankylosing - immunology ; Spondyloarthritis ; T cell receptors ; T-Lymphocytes - immunology ; Th17 Cells - immunology ; Transcriptome ; Young Adult</subject><ispartof>Arthritis &amp; rheumatology (Hoboken, N.J.), 2016-04, Vol.68 (4), p.901-914</ispartof><rights>2016 The Authors. Arthritis &amp; Rheumatology published by Wiley Periodicals, Inc. on behalf of the American College of Rheumatology.</rights><rights>2016, American College of Rheumatology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4765-f5cbe25668ee679c9744d83761b31a99a63c1060a4fd0eed4c5db520e32c86e93</citedby><cites>FETCH-LOGICAL-c4765-f5cbe25668ee679c9744d83761b31a99a63c1060a4fd0eed4c5db520e32c86e93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26841353$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ridley, Anna</creatorcontrib><creatorcontrib>Hatano, Hiroko</creatorcontrib><creatorcontrib>Wong‐Baeza, Isabel</creatorcontrib><creatorcontrib>Shaw, Jacqueline</creatorcontrib><creatorcontrib>Matthews, Katherine K.</creatorcontrib><creatorcontrib>Al‐Mossawi, Hussein</creatorcontrib><creatorcontrib>Ladell, Kristin</creatorcontrib><creatorcontrib>Price, David A.</creatorcontrib><creatorcontrib>Bowness, Paul</creatorcontrib><creatorcontrib>Kollnberger, Simon</creatorcontrib><title>Activation‐Induced Killer Cell Immunoglobulin‐like Receptor 3DL2 Binding to HLA–B27 Licenses Pathogenic T Cell Differentiation in Spondyloarthritis</title><title>Arthritis &amp; rheumatology (Hoboken, N.J.)</title><addtitle>Arthritis Rheumatol</addtitle><description>Objective In the spondyloarthritides (SpA), increased numbers of CD4+ T cells express killer cell immunoglobulin‐like receptor 3DL2 (KIR‐3DL2). The aim of this study was to determine the factors that induce KIR‐3DL2 expression, and to characterize the relationship between HLA–B27 and the phenotype and function of KIR‐3DL2–expressing CD4+ T cells in SpA. Methods In total, 34 B27+ patients with SpA, 28 age‐ and sex‐matched healthy controls (20 B27− and 8 B27+), and 9 patients with rheumatoid arthritis were studied. KIR-3DL2 expression and other phenotypic characteristics of peripheral blood and synovial fluid CD4+ T cells were studied by flow cytometry, quantitative polymerase chain reaction, and Western blotting. T cell receptor clonality was determined by template‐switch anchored reverse transcription–polymerase chain reaction and sequencing analysis. Cytokines were measured by enzyme‐linked immunosorbent assay. Results Cellular activation induced KIR‐3DL2 expression on both naive and effector CD4+ T cells. KIR‐3DL2 binding to B27+ cells promoted expression of KIR‐3DL2, the Th17‐specific transcription factor retinoic acid receptor–related orphan nuclear receptor γt, and the antiapoptotic factor B cell lymphoma 2. KIR‐3DL2+CD4+ T cells in patients with ankylosing spondylitis were oligoclonal and enriched for markers of T cell activation and for the gut homing receptor CCR9. In the presence of B27+ antigen‐presenting cells, KIR‐3DL2+CD4+ T cells produced less interleukin‐2 (IL‐2) but more IL‐17. This effect was blocked by HC10, an antibody that inhibits the binding of KIR‐3DL2 to B27 heavy chains. Conclusion KIR‐3DL2 binding to HLA–B27 licenses Th17 cell differentiation in SpA. These findings raise the therapeutic potential of targeting HLA–B27–KIR‐3DL2 interactions for the treatment of B27+ patients with SpA.</description><subject>Adult</subject><subject>Aged</subject><subject>Arthritis, Psoriatic - immunology</subject><subject>Arthritis, Reactive - immunology</subject><subject>Arthritis, Rheumatoid - immunology</subject><subject>Blotting, Western</subject><subject>Case-Control Studies</subject><subject>CD4-Positive T-Lymphocytes - immunology</subject><subject>Cell Differentiation - immunology</subject><subject>Cytokines - immunology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>HLA-B27 Antigen - immunology</subject><subject>HLA-B27 Antigen - metabolism</subject><subject>Humans</subject><subject>Immunoglobulins</subject><subject>Lymphocytes</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Polymerase chain reaction</subject><subject>Receptors, Antigen, T-Cell - genetics</subject><subject>Receptors, KIR3DL2 - immunology</subject><subject>Receptors, KIR3DL2 - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Spondylarthropathies - immunology</subject><subject>Spondylitis, Ankylosing - immunology</subject><subject>Spondyloarthritis</subject><subject>T cell receptors</subject><subject>T-Lymphocytes - immunology</subject><subject>Th17 Cells - immunology</subject><subject>Transcriptome</subject><subject>Young Adult</subject><issn>2326-5191</issn><issn>2326-5205</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><recordid>eNqNktFqFDEYhQdRbFl74QtIwBu92HaSTJKZG2G7Vbu4oNT1OmQy_-ymZpJtMlPZuz5CoVe-Xp_EbLctKgjmJoH_4-Q_h5NlL3F-iPOcHKnQH9KKYfYk2yeU8DEjOXv68MYV3ssOYjzP06lEznP2PNsjvCwwZXQ_-znRvblUvfHu9up65ppBQ4M-GWshoClYi2ZdNzi_tL4erNlC1nwHdAYa1r0PiJ7MCTo2rjFuiXqPTueT26ubYyLQ3GhwESL6ovqVX4IzGi12miembSGA683dz8g49HXtXbOxPtlZBdOb-CJ71iob4eD-HmXfPrxfTE_H888fZ9PJfKwLwdm4ZboGwjgvAbiodCWKoimp4LimWFWV4lTjZFsVbZMDNIVmTZ0iAkp0yaGio-zdTnc91B00aec-KCvXwXQqbKRXRv45cWYll_5SFiVjPMU4yt7cCwR_MUDsZWeiTjaVAz9EiUXJCC0xzf8DFaIoBStIQl__hZ77IbiUxJbiVGDGtoJvd5QOPsYA7ePeOJfbesiUp7yrR2Jf_W70kXwoQwKOdsAPY2HzbyU5OVvsJH8BxV7IKQ</recordid><startdate>201604</startdate><enddate>201604</enddate><creator>Ridley, Anna</creator><creator>Hatano, Hiroko</creator><creator>Wong‐Baeza, Isabel</creator><creator>Shaw, Jacqueline</creator><creator>Matthews, Katherine K.</creator><creator>Al‐Mossawi, Hussein</creator><creator>Ladell, Kristin</creator><creator>Price, David A.</creator><creator>Bowness, Paul</creator><creator>Kollnberger, Simon</creator><general>Wiley Subscription Services, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7T5</scope><scope>7TM</scope><scope>7U7</scope><scope>C1K</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201604</creationdate><title>Activation‐Induced Killer Cell Immunoglobulin‐like Receptor 3DL2 Binding to HLA–B27 Licenses Pathogenic T Cell Differentiation in Spondyloarthritis</title><author>Ridley, Anna ; Hatano, Hiroko ; Wong‐Baeza, Isabel ; Shaw, Jacqueline ; Matthews, Katherine K. ; Al‐Mossawi, Hussein ; Ladell, Kristin ; Price, David A. ; Bowness, Paul ; Kollnberger, Simon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4765-f5cbe25668ee679c9744d83761b31a99a63c1060a4fd0eed4c5db520e32c86e93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Arthritis, Psoriatic - immunology</topic><topic>Arthritis, Reactive - immunology</topic><topic>Arthritis, Rheumatoid - immunology</topic><topic>Blotting, Western</topic><topic>Case-Control Studies</topic><topic>CD4-Positive T-Lymphocytes - immunology</topic><topic>Cell Differentiation - immunology</topic><topic>Cytokines - immunology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>HLA-B27 Antigen - immunology</topic><topic>HLA-B27 Antigen - metabolism</topic><topic>Humans</topic><topic>Immunoglobulins</topic><topic>Lymphocytes</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Polymerase chain reaction</topic><topic>Receptors, Antigen, T-Cell - genetics</topic><topic>Receptors, KIR3DL2 - immunology</topic><topic>Receptors, KIR3DL2 - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Spondylarthropathies - immunology</topic><topic>Spondylitis, Ankylosing - immunology</topic><topic>Spondyloarthritis</topic><topic>T cell receptors</topic><topic>T-Lymphocytes - immunology</topic><topic>Th17 Cells - immunology</topic><topic>Transcriptome</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ridley, Anna</creatorcontrib><creatorcontrib>Hatano, Hiroko</creatorcontrib><creatorcontrib>Wong‐Baeza, Isabel</creatorcontrib><creatorcontrib>Shaw, Jacqueline</creatorcontrib><creatorcontrib>Matthews, Katherine K.</creatorcontrib><creatorcontrib>Al‐Mossawi, Hussein</creatorcontrib><creatorcontrib>Ladell, Kristin</creatorcontrib><creatorcontrib>Price, David A.</creatorcontrib><creatorcontrib>Bowness, Paul</creatorcontrib><creatorcontrib>Kollnberger, Simon</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>Wiley Online Library (Open Access Collection)</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Arthritis &amp; rheumatology (Hoboken, N.J.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ridley, Anna</au><au>Hatano, Hiroko</au><au>Wong‐Baeza, Isabel</au><au>Shaw, Jacqueline</au><au>Matthews, Katherine K.</au><au>Al‐Mossawi, Hussein</au><au>Ladell, Kristin</au><au>Price, David A.</au><au>Bowness, Paul</au><au>Kollnberger, Simon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Activation‐Induced Killer Cell Immunoglobulin‐like Receptor 3DL2 Binding to HLA–B27 Licenses Pathogenic T Cell Differentiation in Spondyloarthritis</atitle><jtitle>Arthritis &amp; rheumatology (Hoboken, N.J.)</jtitle><addtitle>Arthritis Rheumatol</addtitle><date>2016-04</date><risdate>2016</risdate><volume>68</volume><issue>4</issue><spage>901</spage><epage>914</epage><pages>901-914</pages><issn>2326-5191</issn><eissn>2326-5205</eissn><abstract>Objective In the spondyloarthritides (SpA), increased numbers of CD4+ T cells express killer cell immunoglobulin‐like receptor 3DL2 (KIR‐3DL2). The aim of this study was to determine the factors that induce KIR‐3DL2 expression, and to characterize the relationship between HLA–B27 and the phenotype and function of KIR‐3DL2–expressing CD4+ T cells in SpA. Methods In total, 34 B27+ patients with SpA, 28 age‐ and sex‐matched healthy controls (20 B27− and 8 B27+), and 9 patients with rheumatoid arthritis were studied. KIR-3DL2 expression and other phenotypic characteristics of peripheral blood and synovial fluid CD4+ T cells were studied by flow cytometry, quantitative polymerase chain reaction, and Western blotting. T cell receptor clonality was determined by template‐switch anchored reverse transcription–polymerase chain reaction and sequencing analysis. Cytokines were measured by enzyme‐linked immunosorbent assay. Results Cellular activation induced KIR‐3DL2 expression on both naive and effector CD4+ T cells. KIR‐3DL2 binding to B27+ cells promoted expression of KIR‐3DL2, the Th17‐specific transcription factor retinoic acid receptor–related orphan nuclear receptor γt, and the antiapoptotic factor B cell lymphoma 2. KIR‐3DL2+CD4+ T cells in patients with ankylosing spondylitis were oligoclonal and enriched for markers of T cell activation and for the gut homing receptor CCR9. In the presence of B27+ antigen‐presenting cells, KIR‐3DL2+CD4+ T cells produced less interleukin‐2 (IL‐2) but more IL‐17. This effect was blocked by HC10, an antibody that inhibits the binding of KIR‐3DL2 to B27 heavy chains. Conclusion KIR‐3DL2 binding to HLA–B27 licenses Th17 cell differentiation in SpA. These findings raise the therapeutic potential of targeting HLA–B27–KIR‐3DL2 interactions for the treatment of B27+ patients with SpA.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>26841353</pmid><doi>10.1002/art.39515</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record>
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subjects Adult
Aged
Arthritis, Psoriatic - immunology
Arthritis, Reactive - immunology
Arthritis, Rheumatoid - immunology
Blotting, Western
Case-Control Studies
CD4-Positive T-Lymphocytes - immunology
Cell Differentiation - immunology
Cytokines - immunology
Enzyme-Linked Immunosorbent Assay
Female
Flow Cytometry
HLA-B27 Antigen - immunology
HLA-B27 Antigen - metabolism
Humans
Immunoglobulins
Lymphocytes
Male
Middle Aged
Polymerase chain reaction
Receptors, Antigen, T-Cell - genetics
Receptors, KIR3DL2 - immunology
Receptors, KIR3DL2 - metabolism
Reverse Transcriptase Polymerase Chain Reaction
Spondylarthropathies - immunology
Spondylitis, Ankylosing - immunology
Spondyloarthritis
T cell receptors
T-Lymphocytes - immunology
Th17 Cells - immunology
Transcriptome
Young Adult
title Activation‐Induced Killer Cell Immunoglobulin‐like Receptor 3DL2 Binding to HLA–B27 Licenses Pathogenic T Cell Differentiation in Spondyloarthritis
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