Cathepsin S Contributes to the Pathogenesis of Muscular Dystrophy in Mice

• Published in: The Journal of biological chemistry 2016-05, Vol.291 (19), p.9920-9928
• Main Authors: Tjondrokoesoemo, Andoria, Schips, Tobias G., Sargent, Michelle A., Vanhoutte, Davy, Kanisicak, Onur, Prasad, Vikram, Lin, Suh-Chin J., Maillet, Marjorie, Molkentin, Jeffery D.
• Format: Article
• Language: English
• Subjects: Animals; cathepsin S; Cathepsins - biosynthesis; Cytoskeleton - enzymology; Cytoskeleton - genetics; Cytoskeleton - pathology; dystrophin; Gene Expression Regulation, Developmental; Mice; Mice, Inbred mdx; Mice, Knockout; Molecular Bases of Disease; Muscle Fibers, Skeletal - enzymology; Muscle Fibers, Skeletal - pathology; muscular dystrophy; Muscular Dystrophy, Animal - enzymology; Muscular Dystrophy, Animal - genetics; Muscular Dystrophy, Animal - pathology; Muscular Dystrophy, Duchenne - enzymology; Muscular Dystrophy, Duchenne - genetics; Muscular Dystrophy, Duchenne - pathology; Necrosis; protease; Proteolysis; Sarcolemma - enzymology; Sarcolemma - genetics; Sarcolemma - pathology; skeletal muscle; transgenic mice
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M116.719054

Duchenne muscular dystrophy (DMD) is an X-linked recessive disease caused by mutations in the gene encoding dystrophin. Loss of dystrophin protein compromises the stability of the sarcolemma membrane surrounding each muscle cell fiber, leading to membrane ruptures and leakiness that induces myofiber necrosis, a subsequent inflammatory response, and progressive tissue fibrosis with loss of functional capacity. Cathepsin S (Ctss) is a cysteine protease that is actively secreted in areas of tissue injury and ongoing inflammation, where it participates in extracellular matrix remodeling and healing. Here we show significant induction of Ctss expression and proteolytic activity following acute muscle injury or in muscle from mdx mice, a model of DMD. To examine the functional ramifications associated with greater Ctss expression, the Ctss gene was deleted in the mdx genetic background, resulting in protection from muscular dystrophy pathogenesis that included reduced myofiber turnover and histopathology, reduced fibrosis, and improved running capacity. Mechanistically, deletion of the Ctss gene in the mdx background significantly increased myofiber sarcolemmal membrane stability with greater expression and membrane localization of utrophin, integrins, and β-dystroglycan, which anchor the membrane to the basal lamina and underlying cytoskeletal proteins. Consistent with these results, skeletal muscle-specific transgenic mice overexpressing Ctss showed increased myofiber necrosis, muscle histopathology, and a functional deficit reminiscent of muscular dystrophy. Hence, Ctss induction during muscular dystrophy is a pathologic event that partially underlies disease pathogenesis, and its inhibition might serve as a new therapeutic strategy in DMD.