The RNA Helicase eIF4A Is Required for Sapovirus Translation

The eukaryotic initiation factor 4A (eIF4A) is a DEAD box helicase that unwinds RNA structure in the 5' untranslated region (UTR) of mRNAs. Here, we investigated the role of eIF4A in porcine sapovirus VPg-dependent translation. Using inhibitors and dominant-negative mutants, we found that eIF4A...

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Bibliographic Details
Published in:Journal of virology 2016-05, Vol.90 (10), p.5200-5204
Main Authors: Hosmillo, Myra, Sweeney, Trevor R, Chaudhry, Yasmin, Leen, Eoin, Curry, Stephen, Goodfellow, Ian, Cho, Kyoung-Oh
Format: Article
Language:English
Subjects:
5' Untranslated Regions
Animals
Eukaryotic Initiation Factor-4A - genetics
Eukaryotic Initiation Factor-4A - metabolism
Genome, Viral
Mutation
Protein Binding
Rabbits
Reticulocytes - metabolism
RNA, Viral - metabolism
Sapovirus - genetics
Sapovirus - physiology
Sterols - pharmacology
Swine
Viral Proteins - biosynthesis
Viral Proteins - genetics
Virus Replication
Virus-Cell Interactions
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Summary:The eukaryotic initiation factor 4A (eIF4A) is a DEAD box helicase that unwinds RNA structure in the 5' untranslated region (UTR) of mRNAs. Here, we investigated the role of eIF4A in porcine sapovirus VPg-dependent translation. Using inhibitors and dominant-negative mutants, we found that eIF4A is required for viral translation and infectivity, suggesting that despite the presence of a very short 5' UTR, eIF4A is required to unwind RNA structure in the sapovirus genome to facilitate virus translation.
ISSN:0022-538X
1098-5514
DOI:10.1128/JVI.03174-15