Dopamine D1 receptor expression is bipolar cell type-specific in the mouse retina

• Published in: Journal of comparative neurology (1911) 2016-07, Vol.524 (10), p.2059-2079
• Main Authors: Farshi, Pershang, Fyk-Kolodziej, Bozena, Krolewski, David M., Walker, Paul D., Ichinose, Tomomi
• Format: Article
• Language: English
• Subjects: Amacrine Cells - metabolism; Animals; BAC transgenic mice; Biotin - analogs & derivatives; Biotin - metabolism; Choline O-Acetyltransferase - metabolism; Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels - metabolism; immunohistochemistry; in situ hybridization: RRID: AB_10000347; Luminescent Proteins - genetics; Luminescent Proteins - metabolism; Mice; Mice, Inbred C57BL; Mice, Transgenic; Nerve Tissue Proteins - metabolism; Opsins - metabolism; Potassium Channels - metabolism; Receptors, Dopamine D1 - genetics; Receptors, Dopamine D1 - metabolism; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Retina - cytology; Retinal Bipolar Cells - classification; Retinal Bipolar Cells - cytology; Retinal Bipolar Cells - metabolism; RNA, Messenger - metabolism; RRID: AB_10013483; RRID: AB_10013783; RRID: AB_2079751; RRID: AB_2086774; RRID: AB_2094841; RRID: AB_2115181; RRID: AB_2158332; RRID: AB_2201528; RRID: AB_2248534; RRID: AB_2261205; RRID: AB_2313634; RRID: AB_2314280; RRID: AB_2314947; RRID: AB_397957; RRID: AB_628142; RRID: AB_94936; Synaptotagmin II - metabolism; Transcription Factors - metabolism; Visual Pathways - physiology
• ISSN: 0021-9967; 1096-9861
• DOI: 10.1002/cne.23932

ABSTRACT In the retina, dopamine is a key molecule for daytime vision. Dopamine is released by retinal dopaminergic amacrine cells and transmits signaling either by conventional synaptic or by volume transmission. By means of volume transmission, dopamine modulates all layers of retinal neurons; however, it is not well understood how dopamine modulates visual signaling pathways in bipolar cells. Here we analyzed Drd1a‐tdTomato BAC transgenic mice and found that the dopamine D1 receptor (D1R) is expressed in retinal bipolar cells in a type‐dependent manner. Strong tdTomato fluorescence was detected in the inner nuclear layer and localized to type 1, 3b, and 4 OFF bipolar cells and type 5‐2, XBC, 6, and 7 ON bipolar cells. In contrast, type 2, 3a, 5‐1, 9, and rod bipolar cells did not express Drd1a‐tdTomato. Other interneurons were also found to express tdTomato including horizontal cells and a subset (25%) of AII amacrine cells. Diverse visual processing pathways, such as color or motion‐coded pathways, are thought to be initiated in retinal bipolar cells. Our results indicate that dopamine sculpts bipolar cell performance in a type‐dependent manner to facilitate daytime vision. J. Comp. Neurol. 524:2059–2079, 2016. © 2015 Wiley Periodicals, Inc. Using Drd1a‐tdTomato BAC transgenic mice, authors investigated dopamine D1 receptor localization in retinal bipolar cells. Both ON and OFF bipolar cells express D1 receptors in a type‐dependent manner.