CYC1 Predicts Poor Prognosis in Patients with Breast Cancer

Cytochrome c-1 (CYC1) is an important subunit of mitochondrial complex III. However, its role in tumor progression is unclear. We found that CYC1 was upregulated in breast tumor tissues, especially in tissues with lymph node metastasis. And higher expression of CYC1 correlates with poor prognosis in...

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Bibliographic Details
Published in:Disease markers 2016-01, Vol.2016 (2016), p.1-9
Main Authors: Gao, Qinglei, Wu, Peng, Ma, Ding, Liu, Jia, Gao, Peipei, Gong, Song, Zhang, Zeyu, Zhao, Meisong, Sun, Shujuan, Han, Yingyan, Zhou, Jianfeng
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
Adult
AMP-Activated Protein Kinases - metabolism
Biomarkers, Tumor - genetics
Biomarkers, Tumor - metabolism
Breast cancer
Breast Neoplasms - metabolism
Breast Neoplasms - pathology
Cancer patients
Carcinoma, Ductal - metabolism
Carcinoma, Ductal - pathology
Cell Line, Tumor
Cell Proliferation
Cytochrome b
Cytochromes c1 - genetics
Cytochromes c1 - metabolism
Development and progression
Female
Humans
Medical research
Medicine, Experimental
Metastasis
Middle Aged
Neoplasm Metastasis
Online databases
Oxidative Phosphorylation
Prognosis
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Summary:Cytochrome c-1 (CYC1) is an important subunit of mitochondrial complex III. However, its role in tumor progression is unclear. We found that CYC1 was upregulated in breast tumor tissues, especially in tissues with lymph node metastasis. And higher expression of CYC1 correlates with poor prognosis in breast cancer patients using online databases and tools. Then we confirmed that CYC1 contributed to metastasis and proliferation in two highly metastatic human breast cancer cell lines. Digging into the biological function of CYC1, we found the activity of mitochondrial complex III decreased due to silencing CYC1. Then the ratio of AMP to ATP increased and AMPK was activated. Analyzing units of other mitochondrial complexes, we did not find knockdown of CYC1 expression reduced expression of any other unit of OXPHOS. We concluded that CYC1 promoted tumor metastasis via suppressing activation of AMPK and contributed to tumor growth via facilitating production of ATP. Our results indicated that CYC1 plays crucial roles in breast cancer progression and might be a predictive factor assisting future patient diagnosis.
ISSN:0278-0240
1875-8630
DOI:10.1155/2016/3528064