Three-dimensional paper-based slip device for one-step point-of-care testing

In this study, we developed a new type of paper-based analytical device (PAD), the three-dimensional (3D) slip-PAD, to detect infectious human norovirus for global healthcare. The 3D configuration of the papers combined with a slip design provides unique features and versatility that overcome the li...

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Bibliographic Details
Published in:Scientific reports 2016-05, Vol.6 (1), p.25710-25710, Article 25710
Main Authors: Han, Kwi Nam, Choi, Jong-Soon, Kwon, Joseph
Format: Article
Language:English
Subjects:
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Humanities and Social Sciences
multidisciplinary
Science
Science (multidisciplinary)
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Summary:In this study, we developed a new type of paper-based analytical device (PAD), the three-dimensional (3D) slip-PAD, to detect infectious human norovirus for global healthcare. The 3D configuration of the papers combined with a slip design provides unique features and versatility that overcome the limitations of fluidic manipulation and sensitivity in point-of-care (POC) tests. The assay can be carried out in a single step based on a moveable slip design, making it suitable for unskilled users. The 3D fluidic network developed by layered construction of wax-patterned papers provides different fluidic paths for the sequential delivery of multiple fluids without the need for peripheral equipment. The release and mixing of enhancement reagents on the device improved the sensitivity and detection limit. The assay results could be visualized by naked eye within 10 min, with subsequent amplification of the signal over time (
ISSN:2045-2322
2045-2322
DOI:10.1038/srep25710