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Abundant cytomegalovirus (CMV) reactive clonotypes in the CD8+ T cell receptor alpha repertoire following allogeneic transplantation

Summary Allogeneic stem cell transplantation is potentially curative, but associated with post‐transplantation complications, including cytomegalovirus (CMV) infections. An effective immune response requires T cells recognizing CMV epitopes via their T cell receptors (TCRs). Little is known about th...

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Published in:Clinical and experimental immunology 2016-06, Vol.184 (3), p.389-402
Main Authors: Link, C. S., Eugster, A., Heidenreich, F., Rücker‐Braun, E., Schmiedgen, M., Oelschlägel, U., Kühn, D., Dietz, S., Fuchs, Y., Dahl, A., Domingues, A. M. J., Klesse, C., Schmitz, M., Ehninger, G., Bornhäuser, M., Schetelig, J., Bonifacio, E.
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cited_by cdi_FETCH-LOGICAL-c4430-7a04ef251e024af809912af9c8772b69bab2adc8e236bc1e441314165c46e5f63
cites cdi_FETCH-LOGICAL-c4430-7a04ef251e024af809912af9c8772b69bab2adc8e236bc1e441314165c46e5f63
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container_issue 3
container_start_page 389
container_title Clinical and experimental immunology
container_volume 184
creator Link, C. S.
Eugster, A.
Heidenreich, F.
Rücker‐Braun, E.
Schmiedgen, M.
Oelschlägel, U.
Kühn, D.
Dietz, S.
Fuchs, Y.
Dahl, A.
Domingues, A. M. J.
Klesse, C.
Schmitz, M.
Ehninger, G.
Bornhäuser, M.
Schetelig, J.
Bonifacio, E.
description Summary Allogeneic stem cell transplantation is potentially curative, but associated with post‐transplantation complications, including cytomegalovirus (CMV) infections. An effective immune response requires T cells recognizing CMV epitopes via their T cell receptors (TCRs). Little is known about the TCR repertoire, in particular the TCR‐α repertoire and its clinical relevance in patients following stem cell transplantation. Using next‐generation sequencing we examined the TCR‐α repertoire of CD8+ T cells and CMV‐specific CD8+ T cells in four patients. Additionally, we performed single‐cell TCR‐αβ sequencing of CMV‐specific CD8+ T cells. The TCR‐α composition of human leucocyte antigen (HLA)‐A*0201 CMVpp65– and CMVIE‐specific T cells was oligoclonal and defined by few dominant clonotypes. Frequencies of single clonotypes reached up to 11% of all CD8+ T cells and half of the total CD8+ T cell repertoire was dominated by few CMV‐reactive clonotypes. Some TCR‐α clonotypes were shared between patients. Gene expression of the circulating CMV‐specific CD8+ T cells was consistent with chronically activated effector memory T cells. The CD8+ T cell response to CMV reactivation resulted in an expansion of a few TCR‐α clonotypes to dominate the CD8+ repertoires. These results warrant further larger studies to define the ability of oligoclonally expanded T cell clones to achieve an effective anti‐viral T cell response in this setting.
doi_str_mv 10.1111/cei.12770
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S. ; Eugster, A. ; Heidenreich, F. ; Rücker‐Braun, E. ; Schmiedgen, M. ; Oelschlägel, U. ; Kühn, D. ; Dietz, S. ; Fuchs, Y. ; Dahl, A. ; Domingues, A. M. J. ; Klesse, C. ; Schmitz, M. ; Ehninger, G. ; Bornhäuser, M. ; Schetelig, J. ; Bonifacio, E.</creator><creatorcontrib>Link, C. S. ; Eugster, A. ; Heidenreich, F. ; Rücker‐Braun, E. ; Schmiedgen, M. ; Oelschlägel, U. ; Kühn, D. ; Dietz, S. ; Fuchs, Y. ; Dahl, A. ; Domingues, A. M. J. ; Klesse, C. ; Schmitz, M. ; Ehninger, G. ; Bornhäuser, M. ; Schetelig, J. ; Bonifacio, E.</creatorcontrib><description>Summary Allogeneic stem cell transplantation is potentially curative, but associated with post‐transplantation complications, including cytomegalovirus (CMV) infections. An effective immune response requires T cells recognizing CMV epitopes via their T cell receptors (TCRs). Little is known about the TCR repertoire, in particular the TCR‐α repertoire and its clinical relevance in patients following stem cell transplantation. Using next‐generation sequencing we examined the TCR‐α repertoire of CD8+ T cells and CMV‐specific CD8+ T cells in four patients. Additionally, we performed single‐cell TCR‐αβ sequencing of CMV‐specific CD8+ T cells. The TCR‐α composition of human leucocyte antigen (HLA)‐A*0201 CMVpp65– and CMVIE‐specific T cells was oligoclonal and defined by few dominant clonotypes. Frequencies of single clonotypes reached up to 11% of all CD8+ T cells and half of the total CD8+ T cell repertoire was dominated by few CMV‐reactive clonotypes. Some TCR‐α clonotypes were shared between patients. Gene expression of the circulating CMV‐specific CD8+ T cells was consistent with chronically activated effector memory T cells. The CD8+ T cell response to CMV reactivation resulted in an expansion of a few TCR‐α clonotypes to dominate the CD8+ repertoires. 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S.</creatorcontrib><creatorcontrib>Eugster, A.</creatorcontrib><creatorcontrib>Heidenreich, F.</creatorcontrib><creatorcontrib>Rücker‐Braun, E.</creatorcontrib><creatorcontrib>Schmiedgen, M.</creatorcontrib><creatorcontrib>Oelschlägel, U.</creatorcontrib><creatorcontrib>Kühn, D.</creatorcontrib><creatorcontrib>Dietz, S.</creatorcontrib><creatorcontrib>Fuchs, Y.</creatorcontrib><creatorcontrib>Dahl, A.</creatorcontrib><creatorcontrib>Domingues, A. M. J.</creatorcontrib><creatorcontrib>Klesse, C.</creatorcontrib><creatorcontrib>Schmitz, M.</creatorcontrib><creatorcontrib>Ehninger, G.</creatorcontrib><creatorcontrib>Bornhäuser, M.</creatorcontrib><creatorcontrib>Schetelig, J.</creatorcontrib><creatorcontrib>Bonifacio, E.</creatorcontrib><title>Abundant cytomegalovirus (CMV) reactive clonotypes in the CD8+ T cell receptor alpha repertoire following allogeneic transplantation</title><title>Clinical and experimental immunology</title><addtitle>Clin Exp Immunol</addtitle><description>Summary Allogeneic stem cell transplantation is potentially curative, but associated with post‐transplantation complications, including cytomegalovirus (CMV) infections. An effective immune response requires T cells recognizing CMV epitopes via their T cell receptors (TCRs). Little is known about the TCR repertoire, in particular the TCR‐α repertoire and its clinical relevance in patients following stem cell transplantation. Using next‐generation sequencing we examined the TCR‐α repertoire of CD8+ T cells and CMV‐specific CD8+ T cells in four patients. Additionally, we performed single‐cell TCR‐αβ sequencing of CMV‐specific CD8+ T cells. The TCR‐α composition of human leucocyte antigen (HLA)‐A*0201 CMVpp65– and CMVIE‐specific T cells was oligoclonal and defined by few dominant clonotypes. Frequencies of single clonotypes reached up to 11% of all CD8+ T cells and half of the total CD8+ T cell repertoire was dominated by few CMV‐reactive clonotypes. Some TCR‐α clonotypes were shared between patients. Gene expression of the circulating CMV‐specific CD8+ T cells was consistent with chronically activated effector memory T cells. The CD8+ T cell response to CMV reactivation resulted in an expansion of a few TCR‐α clonotypes to dominate the CD8+ repertoires. These results warrant further larger studies to define the ability of oligoclonally expanded T cell clones to achieve an effective anti‐viral T cell response in this setting.</description><subject>Aged</subject><subject>allogeneic transplantation</subject><subject>Amino Acid Sequence</subject><subject>Antigens, Viral - genetics</subject><subject>Antigens, Viral - immunology</subject><subject>CD8-Positive T-Lymphocytes - immunology</subject><subject>CD8-Positive T-Lymphocytes - pathology</subject><subject>CD8-Positive T-Lymphocytes - virology</subject><subject>Clone Cells</subject><subject>CMV</subject><subject>Cytomegalovirus</subject><subject>Cytomegalovirus - growth &amp; development</subject><subject>Cytomegalovirus - immunology</subject><subject>Cytomegalovirus Infections - etiology</subject><subject>Cytomegalovirus Infections - genetics</subject><subject>Cytomegalovirus Infections - immunology</subject><subject>Cytomegalovirus Infections - virology</subject><subject>Epitopes - genetics</subject><subject>Epitopes - immunology</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Hematopoietic Stem Cell Transplantation - adverse effects</subject><subject>HLA-A2 Antigen - genetics</subject><subject>HLA-A2 Antigen - immunology</subject><subject>Humans</subject><subject>Immunologic Memory</subject><subject>Leukemia, Lymphocytic, Chronic, B-Cell - immunology</subject><subject>Leukemia, Lymphocytic, Chronic, B-Cell - pathology</subject><subject>Leukemia, Lymphocytic, Chronic, B-Cell - therapy</subject><subject>Leukemia, Myeloid, Acute - immunology</subject><subject>Leukemia, Myeloid, Acute - pathology</subject><subject>Leukemia, Myeloid, Acute - therapy</subject><subject>Lymphocytes</subject><subject>Male</subject><subject>Middle Aged</subject><subject>next‐generation sequencing</subject><subject>Original</subject><subject>Receptors, Antigen, T-Cell, alpha-beta - genetics</subject><subject>Receptors, Antigen, T-Cell, alpha-beta - immunology</subject><subject>Sequence Analysis, DNA</subject><subject>Signal Transduction</subject><subject>Single-Cell Analysis</subject><subject>Stem cells</subject><subject>T cell receptor alpha</subject><subject>T cell receptor repertoire</subject><subject>T cell receptors</subject><subject>Transplantation, Homologous</subject><issn>0009-9104</issn><issn>1365-2249</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNp1kcFrFDEUxoNY7Fo9-A9IwEuLTJtkMpnJpVDGagsVL9VryGTf7KZkkzHJbNm7f7ipW4sK5vJ4vB9fvvc-hN5QckrLOzNgTylrW_IMLWgtmooxLp-jBSFEVpISfohepnRXWiEEe4EOmegIobRboB8Xw-yX2mdsdjlsYKVd2No4J3zcf_52giNok-0WsHHBh7ybIGHrcV4D7j907_EtNuBcwQxMOUSs3bTWpZ0g5mAj4DE4F-6tX5WRCyvwYA3OUfs0ufKtzjb4V-hg1C7B68d6hL5-vLztr6qbL5-u-4ubynBek6rVhMPIGgqEcT12RErK9ChN17ZsEHLQA9NL0wGrxWAocE5ryqloDBfQjKI-Qud73WkeNrA04IsRp6ZoNzruVNBW_T3xdq1WYat417K65UXg-FEghu8zpKw2Nj0cQHsIc1K0lYQ3rJF1Qd_9g96FOfqyXqE62VJRU1aokz1lYkgpwvhkhhL1kK0q2apf2Rb27Z_un8jfYRbgbA_cWwe7_yup_vJ6L_kTw6evyQ</recordid><startdate>201606</startdate><enddate>201606</enddate><creator>Link, C. 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J.</creatorcontrib><creatorcontrib>Klesse, C.</creatorcontrib><creatorcontrib>Schmitz, M.</creatorcontrib><creatorcontrib>Ehninger, G.</creatorcontrib><creatorcontrib>Bornhäuser, M.</creatorcontrib><creatorcontrib>Schetelig, J.</creatorcontrib><creatorcontrib>Bonifacio, E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Clinical and experimental immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Link, C. S.</au><au>Eugster, A.</au><au>Heidenreich, F.</au><au>Rücker‐Braun, E.</au><au>Schmiedgen, M.</au><au>Oelschlägel, U.</au><au>Kühn, D.</au><au>Dietz, S.</au><au>Fuchs, Y.</au><au>Dahl, A.</au><au>Domingues, A. M. J.</au><au>Klesse, C.</au><au>Schmitz, M.</au><au>Ehninger, G.</au><au>Bornhäuser, M.</au><au>Schetelig, J.</au><au>Bonifacio, E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Abundant cytomegalovirus (CMV) reactive clonotypes in the CD8+ T cell receptor alpha repertoire following allogeneic transplantation</atitle><jtitle>Clinical and experimental immunology</jtitle><addtitle>Clin Exp Immunol</addtitle><date>2016-06</date><risdate>2016</risdate><volume>184</volume><issue>3</issue><spage>389</spage><epage>402</epage><pages>389-402</pages><issn>0009-9104</issn><eissn>1365-2249</eissn><abstract>Summary Allogeneic stem cell transplantation is potentially curative, but associated with post‐transplantation complications, including cytomegalovirus (CMV) infections. An effective immune response requires T cells recognizing CMV epitopes via their T cell receptors (TCRs). Little is known about the TCR repertoire, in particular the TCR‐α repertoire and its clinical relevance in patients following stem cell transplantation. Using next‐generation sequencing we examined the TCR‐α repertoire of CD8+ T cells and CMV‐specific CD8+ T cells in four patients. Additionally, we performed single‐cell TCR‐αβ sequencing of CMV‐specific CD8+ T cells. The TCR‐α composition of human leucocyte antigen (HLA)‐A*0201 CMVpp65– and CMVIE‐specific T cells was oligoclonal and defined by few dominant clonotypes. Frequencies of single clonotypes reached up to 11% of all CD8+ T cells and half of the total CD8+ T cell repertoire was dominated by few CMV‐reactive clonotypes. Some TCR‐α clonotypes were shared between patients. Gene expression of the circulating CMV‐specific CD8+ T cells was consistent with chronically activated effector memory T cells. The CD8+ T cell response to CMV reactivation resulted in an expansion of a few TCR‐α clonotypes to dominate the CD8+ repertoires. These results warrant further larger studies to define the ability of oligoclonally expanded T cell clones to achieve an effective anti‐viral T cell response in this setting.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>26800118</pmid><doi>10.1111/cei.12770</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record>
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ispartof Clinical and experimental immunology, 2016-06, Vol.184 (3), p.389-402
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1365-2249
language eng
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source PubMed; Oxford Journals Online
subjects Aged
allogeneic transplantation
Amino Acid Sequence
Antigens, Viral - genetics
Antigens, Viral - immunology
CD8-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes - pathology
CD8-Positive T-Lymphocytes - virology
Clone Cells
CMV
Cytomegalovirus
Cytomegalovirus - growth & development
Cytomegalovirus - immunology
Cytomegalovirus Infections - etiology
Cytomegalovirus Infections - genetics
Cytomegalovirus Infections - immunology
Cytomegalovirus Infections - virology
Epitopes - genetics
Epitopes - immunology
Female
Gene expression
Gene Expression Regulation
Hematopoietic Stem Cell Transplantation - adverse effects
HLA-A2 Antigen - genetics
HLA-A2 Antigen - immunology
Humans
Immunologic Memory
Leukemia, Lymphocytic, Chronic, B-Cell - immunology
Leukemia, Lymphocytic, Chronic, B-Cell - pathology
Leukemia, Lymphocytic, Chronic, B-Cell - therapy
Leukemia, Myeloid, Acute - immunology
Leukemia, Myeloid, Acute - pathology
Leukemia, Myeloid, Acute - therapy
Lymphocytes
Male
Middle Aged
next‐generation sequencing
Original
Receptors, Antigen, T-Cell, alpha-beta - genetics
Receptors, Antigen, T-Cell, alpha-beta - immunology
Sequence Analysis, DNA
Signal Transduction
Single-Cell Analysis
Stem cells
T cell receptor alpha
T cell receptor repertoire
T cell receptors
Transplantation, Homologous
title Abundant cytomegalovirus (CMV) reactive clonotypes in the CD8+ T cell receptor alpha repertoire following allogeneic transplantation
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T22%3A06%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Abundant%20cytomegalovirus%20(CMV)%20reactive%20clonotypes%20in%20the%20CD8+%20T%20cell%20receptor%20alpha%20repertoire%20following%20allogeneic%20transplantation&rft.jtitle=Clinical%20and%20experimental%20immunology&rft.au=Link,%20C.%20S.&rft.date=2016-06&rft.volume=184&rft.issue=3&rft.spage=389&rft.epage=402&rft.pages=389-402&rft.issn=0009-9104&rft.eissn=1365-2249&rft_id=info:doi/10.1111/cei.12770&rft_dat=%3Cproquest_pubme%3E4060241941%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c4430-7a04ef251e024af809912af9c8772b69bab2adc8e236bc1e441314165c46e5f63%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1789716312&rft_id=info:pmid/26800118&rfr_iscdi=true