Changes in posterior scleral collagen microstructure in canine eyes with an ADAMTS10 mutation

• Published in: Molecular vision 2016-05, Vol.22, p.503-517
• Main Authors: Boote, Craig, Palko, Joel R, Sorensen, Thomas, Mohammadvali, Ashkan, Elsheikh, Ahmed, Komáromy, András M, Pan, Xueliang, Liu, Jun
• Format: Article
• Language: English
• Subjects: ADAM Proteins - genetics; Animals; Anisotropy; Collagen - metabolism; Disease Models, Animal; Dog Diseases - genetics; Dog Diseases - pathology; Dogs; Female; Glaucoma, Open-Angle - genetics; Glaucoma, Open-Angle - pathology; Glaucoma, Open-Angle - veterinary; Intraocular Pressure; Male; Mutation; Sclera - metabolism; Sclera - pathology; X-Ray Diffraction
• ISSN: 1090-0535; 1090-0535

We aimed to characterize alterations in the posterior scleral collagen microstructure before detectable disease onset in a canine model of open-angle glaucoma caused by an ADAMTS10 mutation. Collagen orientation, anisotropy degree (proportion of preferentially aligned collagen), and relative density were measured at 0.4 mm spatial resolution using synchrotron wide-angle X-ray scattering. For statistical evaluation of structure parameters, regional averages of the peripapillary and mid-posterior sclera were compared between ADAMTS10 mutant (affected) dogs (n = 3) and age-matched (carrier) controls (n = 3). No marked differences in the general pattern of preferential collagen fibril orientation were noted between the control and affected dogs. The peripapillary sclera of all specimens featured strongly aligned circumferential collagen ringing the optic nerve head. Collagen anisotropy was significantly reduced in the mid-posterior sclera of the affected dogs (carrier: 0.27±0.11; affected: 0.24±0.10; p = 0.032) but was not statistically significantly different in the peripapillary sclera (carrier: 0.46±0.15; affected: 0.45±0.17; p = 0.68). Collagen density was statistically significantly reduced in the affected dogs for the mid-posterior sclera (carrier: 28.1±9.14; affected: 18.3±5.12; p