Imaging Tumor Vascularity and Response to Anti-Angiogenic Therapy Using Gaussia Luciferase

We developed a novel approach to assess tumor vascularity using recombinant Gaussia luciferase (rGluc) protein and bioluminescence imaging. Upon intravenous injection of rGluc followed by its substrate coelenterazine, non-invasive visualization of tumor vascularity by bioluminescence imaging was pos...

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Bibliographic Details
Published in:Scientific reports 2016-05, Vol.6 (1), p.26353-26353, Article 26353
Main Authors: Kantar, Rami S., Lashgari, Ghazal, Tabet, Elie I., Lewandrowski, Grant K., Carvalho, Litia A., Tannous, Bakhos A.
Format: Article
Language:English
Subjects:
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Angiogenesis
Angiogenesis Inhibitors - administration & dosage
Animals
Antibodies
Bioluminescence
Blood vessels
Cell Line, Tumor
Copepoda - enzymology
Humanities and Social Sciences
Humans
Imidazoles - administration & dosage
Intravenous administration
Luciferases - metabolism
Luminescent Agents - administration & dosage
Luminescent Measurements
multidisciplinary
Neoplasm Transplantation
Neoplasms - blood supply
Neoplasms - drug therapy
Neoplasms - metabolism
Neoplasms - pathology
Neurosciences
Phenylurea Compounds - administration & dosage
Pyrazines - administration & dosage
Quinolines - administration & dosage
Recombinant Proteins - administration & dosage
Science
Standard deviation
Treatment Outcome
Tumors
Vascular endothelial growth factor
Visualization
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Description
Summary:We developed a novel approach to assess tumor vascularity using recombinant Gaussia luciferase (rGluc) protein and bioluminescence imaging. Upon intravenous injection of rGluc followed by its substrate coelenterazine, non-invasive visualization of tumor vascularity by bioluminescence imaging was possible. We applied this method for longitudinal monitoring of tumor vascularity in response to the anti-angiogenic drug tivozanib. This simple and sensitive method could be extended to image blood vessels/vasculature in many different fields.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep26353