Bulk-like endocytosis plays an important role in the recycling of insulin granules in pancreatic beta cells

Although bulk endocytosis has been found in a number of neuronal and endocrine cells, the molecular mechanism and physiological function of bulk endocytosis remain elusive. In pancreatic beta cells, we have observed bulk-like endocytosis evoked both by flash photolysis and trains of depolarization....

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Bibliographic Details
Published in:Protein & cell 2012-08, Vol.3 (8), p.618-626
Main Authors: Wen, Du, Xue, Yanhong, Liang, Kuo, Yuan, Tianyi, Lu, Jingze, Zhao, Wei, Xu, Tao, Chen, Liangyi
Format: Article
Language:English
Subjects:
Animals
Biochemistry
Biomedical and Life Sciences
Calcium - metabolism
Cell Biology
Cytoplasmic Granules - metabolism
Developmental Biology
Diabetes Mellitus - metabolism
Diabetes Mellitus - pathology
Disease Models, Animal
Dynamins - metabolism
Electric Capacitance
Endocytosis - physiology
Human Genetics
Insulin - metabolism
Insulin-Secreting Cells - metabolism
Insulin-Secreting Cells - pathology
Life Sciences
Male
Mice
Mice, Inbred C57BL
Patch-Clamp Techniques
Photolysis
Primary Cell Culture
Protein Science
Research Article
Stem Cells
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Summary:Although bulk endocytosis has been found in a number of neuronal and endocrine cells, the molecular mechanism and physiological function of bulk endocytosis remain elusive. In pancreatic beta cells, we have observed bulk-like endocytosis evoked both by flash photolysis and trains of depolarization. Bulk-like endocytosis is a clathrin-independent process that is facilitated by enhanced extracellular Ca 2+ entry and suppressed by the inhibition of dynamin function. Moreover, defects in bulk-like endocytosis are accompanied by hyperinsulinemia in primary beta cells dissociated from diabetic KKAy mice, which suggests that bulk-like endocytosis plays an important role in maintaining the exo-endocytosis balance and beta cell secretory capability.
ISSN:1674-800X
1674-8018
DOI:10.1007/s13238-012-2938-0