Reduced response of human meniscal cells to Osteogenic Protein 1 during osteoarthritis and pro-inflammatory stimulation

Summary Objective Many cell types lose responsiveness to anabolic factors during inflammation and disease. Osteogenic Protein 1 (OP1/BMP7) was evaluated for the ability to enhance extracellular matrix synthesis in healthy and OA meniscus cells. Mechanisms of cell response to OP1 were explored. Desig...

Full description

Saved in:
Bibliographic Details
Published in:Osteoarthritis and cartilage 2016-06, Vol.24 (6), p.1036-1046
Main Authors: Vanderman, K.S, Loeser, R.F, Chubinskaya, S, Anderson, A, Ferguson, C.M
Format: Article
Language:English
Subjects:
Aggrecans
BMP
Bone Morphogenetic Protein 7
Cartilage, Articular
Cells, Cultured
Chondrocytes
Cytokine
Extracellular matrix
Humans
Meniscus
OP1
Osteoarthritis
Rheumatology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Summary Objective Many cell types lose responsiveness to anabolic factors during inflammation and disease. Osteogenic Protein 1 (OP1/BMP7) was evaluated for the ability to enhance extracellular matrix synthesis in healthy and OA meniscus cells. Mechanisms of cell response to OP1 were explored. Design Meniscus and cartilage tissues from healthy tissue donors and osteoarthritis (OA) patients undergoing total knee arthroplasties were acquired. Primary cell cultures were stimulated with OP1 and/or inflammatory factors (IL1α, IL1β, or fibronectin fragments (FnF)) and cellular responses were analyzed by RT-qPCR and immunoblots. Frozen section immunohistochemistry (IHC) was conducted to assess OP1 and receptor proteins in normal and OA meniscus. Results OP1 treatment of normal meniscus cells resulted in significant, dose-dependent increases in ACAN (aggrecan) and COL2A1 , and decreased MMP13 gene transcription, while only ACAN was upregulated ( P  
ISSN:1063-4584
1522-9653
DOI:10.1016/j.joca.2015.12.017