A Sensitive IHC Method for Monitoring Autophagy-Specific Markers in Human Tumor Xenografts

Objective. Use of tyramide signal amplification (TSA) to detect autophagy biomarkers in formalin fixed and paraffin embedded (FFPE) xenograft tissue. Materials and Methods. Autophagy marker regulation was studied in xenograft tissues using Amp HQ IHC and standard IHC methods. Results. The data demon...

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Bibliographic Details
Published in:Journal of biomarkers 2016-01, Vol.2016, p.1-11
Main Authors: He, Helen, Yang, Yu, Xiang, Zhongmin, Yu, Lunyin, Chouitar, Jouhara, Yu, Jie, D’Amore, Natalie Roy, Li, Ping, Li, Zhi, Bowman, Douglas, Theisen, Matthew, Brownell, James E., Tirrell, Stephen
Format: Article
Language:English
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Summary:Objective. Use of tyramide signal amplification (TSA) to detect autophagy biomarkers in formalin fixed and paraffin embedded (FFPE) xenograft tissue. Materials and Methods. Autophagy marker regulation was studied in xenograft tissues using Amp HQ IHC and standard IHC methods. Results. The data demonstrate the feasibility of using high sensitivity TSA IHC assays to measure low abundant autophagy markers in FFPE xenograft tissue.
ISSN:2090-8660
2090-7699
DOI:10.1155/2016/1274603