Overexpression of miR-30a in lung adenocarcinoma A549 cell line inhibits migration and invasion via targeting EYA2

• Published in: Acta biochimica et biophysica Sinica 2016-03, Vol.48 (3), p.220-228
• Main Authors: Yuan, Yuncang, Zheng, Shangyong, Li, Qian, Xiang, Xudong, Gao, Tangxin, Ran, Pengzhan, Sun, Lijuan, Huang, Qionglin, Xie, Fei, Du, Jing, Xiao, Chunjie
• Format: Article
• Language: English
• Subjects: 3' Untranslated Regions; A549细胞; Adenocarcinoma - genetics; Adenocarcinoma - pathology; Cell Line, Tumor; Down-Regulation; Humans; Intracellular Signaling Peptides and Proteins - genetics; Lung Neoplasms - genetics; Lung Neoplasms - pathology; MicroRNAs - genetics; Neoplasm Invasiveness - genetics; Neoplasm Metastasis - genetics; Nuclear Proteins - genetics; Original; Protein Tyrosine Phosphatases - genetics; 侵袭; 细胞增殖; 细胞株; 细胞迁移; 肺腺癌; 荧光定量PCR; 非小细胞肺癌
• ISSN: 1672-9145; 1745-7270
• DOI: 10.1093/abbs/gmv139

Mic：roRNAs （miRNAs） are a class of small non-coding RNAs and closely related to the pathogenesis of cancers. Increasing evidence indicates that miR-30a plays a profound role during the development of cancers. However, the functions of miR-30a in non-small-cell lung cancer （NSCLC） are still ambigu- ous. Here we found that miR-30a was decreased in lung adenocarcinoma A549 cells and in tissue samples from 14 patients by qRT-PCR, and also found that overexpression of miR-30a in A549 cells inhibited migration and invasion but not cell proliferation and： cell cycle progression by wound-healing assay, matrigel invasion assay, MTS-based cell proliferation assay, and flow cytome- try-based cell cycle analysis, respectively. We further explored the potential mechanism of miR-3Oa- mediated gene regulation in lung adenocarcinoma cell lines. EYA2 is a predicted target of miR-30a, and it has been found that EYA2expression is inhibited by miR-30a in breast cancer cells. We demon- strated that EYA2 is a direct target of miR-30a by using the dual-luciferase reporter assay in A549 cells and showed that EYA2 protein levels are inversely correlated with miR-30a expression in A549 and BEAS-2B cells. In addition, we also confirmed the rescue effects of EYA2overexpression in A549 cells by cotransfection with EYA2 expression vector and miR-30a mimics, Taken together, our results demonstrate that overexpression of miR-30a in lung adenocarcinoma A549 cells can inhibit cell mi- gration and invasion, which is partially attributed to the decrease of EYA2 expression. Our findings suggest that miR-30a may be used as a new potential target for the treatment of lung adenocarcinoma in the future.