Luteolin potentiates the effects of aminoglycoside and β-lactam antibiotics against methicillin-resistant Staphylococcus aureus in vitro

Methicillin-resistant Staphylococcus aureus (MRSA) infection has become a serious clinical problem worldwide, and alternative natural or combination drug therapies are required for its treatment. The aim of the present study was to examined the antimicrobial activity of luteolin (LUT) against MRSA....

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Bibliographic Details
Published in:Experimental and therapeutic medicine 2016-06, Vol.11 (6), p.2597-2601
Main Authors: JOUNG, DAE-KI, KANG, OK-HWA, SEO, YUN-SOO, ZHOU, TIAN, LEE, YOUNG-SEOB, HAN, SIN-HEE, MUN, SU-HYUN, KONG, RYONG, SONG, HO-JUN, SHIN, DONG-WON, KWON, DONG-YEUL
Format: Article
Language:English
Subjects:
aminoglycoside antibiotics
Aminoglycosides
Analysis
Antibiotics
Antimicrobial agents
Bacteria
Care and treatment
Drug dosages
Experiments
iuyeolin
methicillin-resistant Staphylococcus aureus
Nosocomial infections
Pathogens
Skin
Staphylococcus aureus infections
Staphylococcus infections
Statistical analysis
Studies
β-lactam
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Summary:Methicillin-resistant Staphylococcus aureus (MRSA) infection has become a serious clinical problem worldwide, and alternative natural or combination drug therapies are required for its treatment. The aim of the present study was to examined the antimicrobial activity of luteolin (LUT) against MRSA. Luteolin is a polyphenolic flavonoid compound with a wide spectrum of biological activities. The antimicrobial activities of LUT and the antibiotics ampicillin (AM), oxacillin (OX) and gentamicin (GT), used alone or in combination, were evaluated against five clinical MRSA isolates and two reference strains using a minimum inhibitory concentration (MIC) assay, MTT colorimetric assay, checkerboard dilution test and time-kill assay. The MIC of LUT against all strains was found to be 62.5 µg/ml. The combinations of LUT and antibiotics exhibited a synergistic effect against MRSA in the majority of cases, as determined by the checkerboard method. Time-kill curves revealed that a combination of LUT with AM, OX or GT significantly reduced bacterial counts, which dropped below the lowest detectable limit after 24 h. These results indicate that LUT potentiates the effects of β-lactam and aminoglycoside antibiotics against MRSA.
ISSN:1792-0981
1792-1015
DOI:10.3892/etm.2016.3212