Structural and functional changes in gap junctional intercellular communication in a rat model of overactive bladder syndrome induced by partial bladder outlet obstruction

The aim of the present study was to investigate the association between connexin (Cx)43 levels and alterations in gap junctional mediation of intercellular communication in overactive bladder syndrome (OAB), and to examine the effects of connexin inhibitor on this condition. Adult female Wistar rats...

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Bibliographic Details
Published in:Experimental and therapeutic medicine 2016-06, Vol.11 (6), p.2139-2146
Main Authors: Zhou, Fenghai, Li, Haiyuan, Zhou, Chuan, Lv, Haidi, Ma, Yulei, Wang, Yangmin, Song, B O
Format: Article
Language:English
Subjects:
Aged men
Allergic reaction
Allergy
Analysis
Bladder
Causes of
Communication
Health aspects
Hypertrophy
Laboratory animals
Microscopy
Pathogenesis
Prostate
Rats as laboratory animals
Risk factors
Rodents
Smooth muscle
Studies
Urinary incontinence
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Summary:The aim of the present study was to investigate the association between connexin (Cx)43 levels and alterations in gap junctional mediation of intercellular communication in overactive bladder syndrome (OAB), and to examine the effects of connexin inhibitor on this condition. Adult female Wistar rats with OAB following partial bladder outlet obstruction (PBBO) (OAB group, n=37) and sham-operated rats (control group, n=17) were studied. The ultrastructure of the rat detrusor was observed by transmission electron microscopy and the protein expression levels of Cx43 were analyzed using western blot analysis. Furthermore, bladder detrusor cells in both groups were cultured and cells in the OAB group were randomly divided into ten groups. In nine of these groups, 18-β glycyrrhetinic acid (18β-GA) was administered at various doses and durations. All groups were compared using fluorescence redistribution after photobleaching and a laser scanning confocal microscope. Cystometry demonstrated that gap junctions were an abundant mechanism among adjacent cells, and Cx43 protein expression levels were increased in the OAB group following 6 weeks of obstruction, as compared with the control group. Mean fluorescence recovery rates in the OAB group were significantly increased, as compared with the control group (P
ISSN:1792-0981
1792-1015
DOI:10.3892/etm.2016.3246