Acoustofluidic Fluorescence Activated Cell Sorter

Selective isolation of cell subpopulations with defined biological characteristics is crucial for many biological studies and clinical applications. In this work, we present the development of an acoustofluidic fluorescence activated cell sorting (FACS) device that simultaneously performs on-demand,...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2015-12, Vol.87 (24), p.12051-12058
Main Authors: Nawaz, Ahmad Ahsan, Chen, Yuchao, Nama, Nitesh, Nissly, Ruth Helmus, Ren, Liqiang, Ozcelik, Adem, Wang, Lin, McCoy, J. Philip, Levine, Stewart J, Huang, Tony Jun
Format: Article
Language:English
Subjects:
Acoustics
Activated
Biological
Cell Survival
Cells
Devices
Electronics
Flow Cytometry - economics
Flow Cytometry - instrumentation
Flow Cytometry - standards
Fluorescence
HeLa Cells
Humans
Purity
Reproducibility of Results
Sorting
Surface acoustic waves
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Summary:Selective isolation of cell subpopulations with defined biological characteristics is crucial for many biological studies and clinical applications. In this work, we present the development of an acoustofluidic fluorescence activated cell sorting (FACS) device that simultaneously performs on-demand, high-throughput, high-resolution cell detection and sorting, integrated onto a single chip. Our acoustofluidic FACS device uses the “microfluidic drifting” technique to precisely focus cells/particles three dimensionally and achieves a flow of single-file particles/cells as they pass through a laser interrogation region. We then utilize short bursts (150 μs) of standing surface acoustic waves (SSAW) triggered by an electronic feedback system to sort fluorescently labeled particles/cells with desired biological properties. We have demonstrated continuous isolation of fluorescently labeled HeLa cells from unlabeled cells at a throughput of ∼1200 events/s with a purity reaching 92.3 ± 3.39%. Furthermore, 99.18% postsort cell viability indicates that our acoustofluidic sorting technique maintains a high integrity of cells. Therefore, our integrated acoustofluidic FACS device is demonstrated to achieve two-way cell sorting with high purity, biocompatibility, and biosafety. We believe that our device has significant potential for use as a low-cost, high-performance, portable, and user-friendly FACS instrument.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.5b02398