Intestinal stem cell growth and differentiation on a tubular scaffold with evaluation in small and large animals

To investigate the growth and differentiation of intestinal stem cells on a novel tubular scaffold and . Intestinal progenitor cells from mice or humans were cultured with myofibroblasts, macrophages and/or bacteria, and evaluated in mice via omental implantation. Mucosal regeneration was evaluated...

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Bibliographic Details
Published in:Regenerative medicine 2016-01, Vol.11 (1), p.45-61
Main Authors: Shaffiey, Shahab A, Jia, Hongpeng, Keane, Timothy, Costello, Cait, Wasserman, Deena, Quidgley, Maria, Dziki, Jenna, Badylak, Stephen, Sodhi, Chhinder P, March, John C, Hackam, David J
Format: Article
Language:English
Subjects:
Animals
artificial intestine
Blood Vessels - pathology
Cell Differentiation
Cell Proliferation
Coculture Techniques
Disease Models, Animal
Dogs
Escherichia coli - physiology
Inflammation - pathology
intestinal stem cells
Intestine, Small - cytology
Lactic Acid - chemistry
Lactobacillus - physiology
Mice, Inbred C57BL
Microvilli - ultrastructure
necrotizing enterocolitis
Polyglycolic Acid - chemistry
scaffold
short bowel syndrome
Stem Cell Niche
Stem Cell Transplantation
Stem Cells - cytology
Tissue Scaffolds - chemistry
tissue-engineered small intestine
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Summary:To investigate the growth and differentiation of intestinal stem cells on a novel tubular scaffold and . Intestinal progenitor cells from mice or humans were cultured with myofibroblasts, macrophages and/or bacteria, and evaluated in mice via omental implantation. Mucosal regeneration was evaluated in dogs after rectal mucosectomy followed by scaffold implantation. Intestinal progenitor cells differentiated into crypt-villi structures on the scaffold. Differentiation and scaffold coverage was enhanced by coculture with myofibroblasts, macrophages and probiotic bacteria, while the implanted scaffolds enhanced mucosal regeneration in the dog rectum. Intestinal stem cell growth and differentiation on a novel tubular scaffold is enhanced through addition of cellular and microbial components, as validated in mice and dogs.
ISSN:1746-0751
1746-076X
DOI:10.2217/rme.15.70