Cyclooxygenase-2 and 5-Lipoxygenase in Dogs with Chronic Enteropathies

Background Cyclooxygenase‐2 (COX‐2) is a key enzyme in the synthesis of pro‐inflammatory prostaglandins and 5‐lipoxygenase (5‐LO) is the major source of leukotrienes. Their role in IBD has been demonstrated in humans and animal models, but not in dogs with chronic enteropathies (CCE). Hypothesis COX...

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Bibliographic Details
Published in:Journal of veterinary internal medicine 2014-11, Vol.28 (6), p.1684-1691
Main Authors: Dumusc, S.D., Ontsouka, E.C., Schnyder, M., Hartnack, S., Albrecht, C., Bruckmaier, R.M., Burgener, I.A.
Format: Article
Language:English
Subjects:
Animals
Arachidonate 5-Lipoxygenase - metabolism
Arachidonate 5-Lipoxygenase - physiology
Biopsy
Case-Control Studies
Chronic Disease
Colon - chemistry
Colon - enzymology
Crohn's disease
Cyclooxygenase 2 - metabolism
Cyclooxygenase 2 - physiology
Cytokines
Diarrhea - enzymology
Diarrhea - veterinary
Dog Diseases - enzymology
Dogs
Duodenum - chemistry
Duodenum - enzymology
Fatty acids
Female
Food-responsive diarrhea
Gastroenterology
Homeostasis
Inflammation
Inflammatory bowel disease
Inflammatory Bowel Diseases - enzymology
Inflammatory Bowel Diseases - veterinary
Interleukin
Interleukin-10 - analysis
Interleukin-1beta - analysis
Interleukin-4 - analysis
Internal medicine
Leukotriene
Male
Prospective Studies
Real-Time Polymerase Chain Reaction
Transforming Growth Factor beta - analysis
Trends
Tumor Necrosis Factor-alpha - analysis
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Summary:Background Cyclooxygenase‐2 (COX‐2) is a key enzyme in the synthesis of pro‐inflammatory prostaglandins and 5‐lipoxygenase (5‐LO) is the major source of leukotrienes. Their role in IBD has been demonstrated in humans and animal models, but not in dogs with chronic enteropathies (CCE). Hypothesis COX‐2 and 5‐LO are upregulated in dogs with CCE. Animals Fifteen healthy control dogs (HCD), 10 dogs with inflammatory bowel disease (IBD), and 15 dogs with food‐responsive diarrhea (FRD). Methods Prospective study. mRNA expression of COX‐2, 5‐LO, IL‐1b, IL‐4, IL‐6, TNF, IL‐10 and TFG‐β was evaluated by quantitative real‐time RT‐PCR in duodenal and colonic biopsies before and after treatment. Results COX‐2 expression in the colon was significantly higher in IBD and FRD before and after treatment (all P 
ISSN:0891-6640
1939-1676
DOI:10.1111/jvim.12463