Haemoglobin J-Baltimore can be detected by HbA1c electropherogram but with underestimated HbA1c value

• Published in: Biochemia medica 2016, Vol.26 (2), p.240-242
• Main Authors: Brunel, Valéry, Lahary, Agnčs, Chagraoui, Abdeslam, Thuillez, Christian
• Format: Article
• Language: English
• Subjects: Adult; capillary electrophoresis; Diabetes, Gestational - blood; Electrophoresis, Capillary; Female; glycated haemoglobin; Glycated Hemoglobin A - isolation & purification; Glycated Hemoglobin A - metabolism; haemoglobin J-Baltimore; Hemoglobin J - isolation & purification; Hemoglobin J - metabolism; Humans; in-Brief; Pregnancy
• ISSN: 1330-0962; 1846-7482; 1846-7482
• DOI: 10.11613/BM.2016.026

Glycated haemoglobin (HbA(1c)) is considered the gold standard for assessing diabetes compensation and treatment. In addition, fortuitous detection of haemoglobin variants during HbA1c measurement is not rare. Recently, two publications reported different conclusions on accuracy of HbA(1c) value using capillary electrophoresis method in presence of haemoglobin J-Baltimore (HbJ).
Here we describe the fortuitous detection of unknown HbJ using capillary electrophoresis for measurement of HbA(1c). A patient followed for gestational diabetes in our laboratory presented unknown haemoglobin on Capillarys 2 Flex Piercing analyser which was identified as HbJ. HbJ is not associated with haematological abnormalities. High Performance Liquid Chromatography methods are known to possibly underestimate HbA(1c) value in the presence of this variant. This variant and its glycated form are clearly distinguished on electropherogram but HbJ was responsible for underestimating the true area of HbA(1c).
 Capillary electrophoresis is a good method for detecting HbJ but does not seem suitable for evaluation of HbA(1C) value in patients in presence of HbJ variant.