Neural transcription factors bias cleavage stage blastomeres to give rise to neural ectoderm

• Published in: Genesis (New York, N.Y. : 2000) N.Y. : 2000), 2016-06, Vol.54 (6), p.334-349
• Main Authors: Gaur, Shailly, Mandelbaum, Max, Herold, Mona, Majumdar, Himani Datta, Neilson, Karen M., Maynard, Thomas M., Mood, Kathy, Daar, Ira O., Moody, Sally A.
• Format: Article
• Language: English
• Subjects: Animals; Anura; Blastomeres - metabolism; Blastula - growth & development; Cell Differentiation - genetics; Ectoderm - growth & development; Ectoderm - metabolism; Forkhead Transcription Factors - biosynthesis; Forkhead Transcription Factors - genetics; Foxd4; Foxd4l1; Foxd4l1.1; Foxd5; Gastrula - growth & development; geminin; Gene Expression Regulation, Developmental; Humans; Mice; neural induction; Neural Plate - growth & development; Neural Plate - metabolism; Sox11; Sox2; Transcription Factors - biosynthesis; Transcription Factors - genetics; Xenopus; Xenopus laevis - genetics; Xenopus laevis - growth & development; Xenopus Proteins - biosynthesis; Xenopus Proteins - genetics; Zic1; Zic2; Zygote - growth & development
• ISSN: 1526-954X; 1526-968X
• DOI: 10.1002/dvg.22943

Summary The decision by embryonic ectoderm to give rise to epidermal versus neural derivatives is the result of signaling events during blastula and gastrula stages. However, there also is evidence in Xenopus that cleavage stage blastomeres contain maternally derived molecules that bias them toward a neural fate. We used a blastomere explant culture assay to test whether maternally deposited transcription factors bias 16‐cell blastomere precursors of epidermal or neural ectoderm to express early zygotic neural genes in the absence of gastrulation interactions or exogenously supplied signaling factors. We found that Foxd4l1, Zic2, Gmnn, and Sox11 each induced explants made from ventral, epidermis‐producing blastomeres to express early neural genes, and that at least some of the Foxd4l1 and Zic2 activities are required at cleavage stages. Similarly, providing extra Foxd4l1 or Zic2 to explants made from dorsal, neural plate‐producing blastomeres significantly increased the expression of early neural genes, whereas knocking down either significantly reduced them. These results show that maternally delivered transcription factors bias cleavage stage blastomeres to a neural fate. We demonstrate that mouse and human homologs of Foxd4l1 have similar functional domains compared to the frog protein, as well as conserved transcriptional activities when expressed in Xenopus embryos and blastomere explants. genesis 54:334–349, 2016. © 2016 Wiley Periodicals, Inc.