Cell-free expression of functional receptor tyrosine kinases

Receptor tyrosine kinases (RTKs) play critical roles in physiological and pathological processes and are important anticancer drug targets. In vitro mechanistic and drug discovery studies of full-length RTKs require protein that is both fully functional and free from contaminating proteins. Here we...

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Bibliographic Details
Published in:Scientific reports 2015-08, Vol.5 (1), p.12896-12896, Article 12896
Main Authors: He, Wei, Scharadin, Tiffany M., Saldana, Matthew, Gellner, Candice, Hoang-Phou, Steven, Takanishi, Christina, Hura, Gregory L., Tainer, John A, Carraway III, Kermit L., Henderson, Paul T., Coleman, Matthew A.
Format: Article
Language:English
Subjects:
631/45/612/1237
692/699/67/1347
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82/80
Apolipoprotein A
Apolipoprotein A-I
BASIC BIOLOGICAL SCIENCES
Cell-Free System
Cyclin-dependent kinases
Drug discovery
Epidermal growth factor receptors
ErbB-2 protein
Humanities and Social Sciences
multidisciplinary
Receptor Protein-Tyrosine Kinases - metabolism
Science
Tyrosine
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Summary:Receptor tyrosine kinases (RTKs) play critical roles in physiological and pathological processes and are important anticancer drug targets. In vitro mechanistic and drug discovery studies of full-length RTKs require protein that is both fully functional and free from contaminating proteins. Here we describe a rapid cell-free and detergent-free co-translation method for producing full-length and functional ERBB2 and EGFR receptor tyrosine kinases supported by water-soluble apolipoprotein A-I based nanolipoprotein particles.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep12896