Boronic acid disk diffusion for the phenotypic detection of polymerase chain reaction-confirmed, carbapenem-resistant, gram-negative bacilli isolates

• Published in: BMC microbiology 2016-07, Vol.16 (1), p.135-135, Article 135
• Main Authors: Elsherif, Rasha, Ismail, Dalia, Elawady, Sanaa, Jastaniah, Samyah, Al-Masaudi, Saad, Harakeh, Steve, Karrouf, Gamal
• Format: Article
• Language: English
• Subjects: Acids; Anti-Bacterial Agents - pharmacology; Bacterial Proteins - metabolism; beta-Lactamases - metabolism; Boronic Acids - administration & dosage; Boronic Acids - chemistry; Boronic Acids - pharmacology; Carbapenems - pharmacology; Care and treatment; Complications and side effects; Disk Diffusion Antimicrobial Tests - methods; Drug Resistance, Multiple, Bacterial; Enzymes; Female; Gram-Negative Bacteria - drug effects; Gram-Negative Bacteria - enzymology; Gram-Negative Bacteria - genetics; Gram-Negative Bacteria - isolation & purification; Gram-Negative Bacterial Infections - microbiology; Hospitals; Humans; Identification; Imipenem - administration & dosage; Imipenem - pharmacology; Klebsiella infections; Male; Performance evaluation; Phenotype; Polymerase chain reaction; Polymerase Chain Reaction - methods; Thienamycins - pharmacology
• ISSN: 1471-2180; 1471-2180
• DOI: 10.1186/s12866-016-0754-z

The Middle East is regarded as a secondary reservoir for OXA-48 and New Delhi metallo-β-lactamase (NDM) carbapenemases. One of the main challenges in clinical microbiology diagnostics is the detection of carbapenemases. For this reason simple screening methods have been sought to detect carbapenemase producers to determine appropriate therapeutic measures and implement infection control interventions. The present study aimed to evaluate the efficacy of the modified Hodge test (MHT) and a boronic acid-based combined disk test using carbapenems as substrates for the phenotypic determination of OXA-48 and NDM type carbapenemases in 45 epidemiologically unrelated carbapenem-resistant clinical isolates of Klebsiella pneumoniae (13 isolates), Acinetobacter baumanii (20 isolates), and Pseudomonas aeruginosa (12 isolates). Boronic acid disk test using meropenem as substrate and 600 µg of 3- aminophenylboronic acid (APB) was the most sensitive method (83.33 %) for detection of OXA-48, while the most specific method was MHT (100 %). As regards NDM carbapenemase, boronic acid disk tests using imipenem and 600 µg of APB per disk, and meropenem with 300 or 600 µg of APB were the most  sensitive methods (87.50 %), while the most specific method was the MHT (100 %). The results of the present study indicate that phenotypic screening with the MHT and the boronic acid disk test may be used to detect OXA-48 and NDM carbapenemases in Gram-negative bacilli clinical isolates, and that these tests can be easily applied in tertiary care settings with minimal infrastructure.